Kato Eiji, Lemler Jeffery, Sakurai Kaoru, Yamada Masahiro
President, Implant and Tissue Engineering Dental Network-Tokyo, Tokyo, Japan; associate professor, Implantology and Periodontics, New York University College of Dentistry, NY, USA; professor and chair, Department of Removable Prosthodontics & Gerodontology, Tokyo Dental College, Chiba, Japan; principal investigator, Implant and Tissue Engineering Dental Network-Tokyo, Tokyo, Japan, and assistant professor, Department of Removable Prosthodontics & Gerodontology, Tokyo Dental College, Chiba, Japan.
Clin Implant Dent Relat Res. 2014 Apr;16(2):202-11. doi: 10.1111/j.1708-8208.2012.00467.x. Epub 2012 Jul 18.
The objective of this study was to compare osteoconductivity and biodegradation properties of an in-house fabricated beta-tricalcium phosphate (b-TCP)-collagen composite with those of Bio-Oss Collagen® (Osteohealth, Shirley, NY, USA) using a rat calvarial critical-size defect model.
b-TCP-collagen composite material was fabricated by mixing b-TCP granules having a particle size of 0.15 to 0.8 mm and 75% porosity, with bovine dermis-derived soluble collagen sponge. The dry weight ratio of b-TCP granules-to-collagen ratios was 4:1. Bio-Oss Collagen or the b-TCP-collagen composite was used to fill a 5.0 mm-diameter calvarial defect in rats. The defects were evaluated by histological and histomorphological analyses of decalcified histological sections with hematoxylin and eosin staining 6 and 10 weeks, respectively, after surgery.
The defect implanted with the b-TCP composite contained immature bone structures with dense connective tissue in contrast to the abundant fibrous tissue, but no trabecular structure was observed within the defect implanted with Bio-Oss Collagen at 6 weeks postoperatively. Eventually, the defect filled with the b-TCP composite was covered with dense, continuous, mature bone tissue with complete replacement of the graft material. However, in defects filled with Bio-Oss Collagen, only dense connective tissue, containing limited amounts of immature trabecular bone and abundant remnant Bio-Oss particles, was observed. Histomorphological analysis revealed that the b-TCP composite caused greater tissue augmentation with a larger volume of bone tissue observed in the defect and greater bioabsorption of remnant material than Bio-Oss Collagen.
These results indicated that the b-TCP composite has greater osteoconductivity and better biodegradation properties than Bio-Oss Collagen; these properties of the b-TCP-collagen composite complimented bone formation and remodeling.
本研究的目的是使用大鼠颅骨临界尺寸缺损模型，比较自制的β-磷酸三钙（β-TCP）-胶原蛋白复合材料与Bio-Oss Collagen®（美国纽约州雪莉市Osteohealth公司）的骨传导性和生物降解特性。
通过将粒径为0.15至0.8毫米、孔隙率为75%的β-TCP颗粒与牛真皮来源的可溶性胶原海绵混合，制备β-TCP-胶原蛋白复合材料。β-TCP颗粒与胶原蛋白的干重比为4:1。使用Bio-Oss Collagen或β-TCP-胶原蛋白复合材料填充大鼠直径5.0毫米的颅骨缺损。分别在术后6周和10周，通过对脱钙组织切片进行苏木精和伊红染色的组织学和组织形态学分析来评估缺损情况。
与富含纤维组织相比，植入β-TCP复合材料的缺损处含有不成熟的骨结构和致密结缔组织，但术后6周，植入Bio-Oss Collagen的缺损处未观察到小梁结构。最终，填充β-TCP复合材料的缺损被致密、连续、成熟的骨组织覆盖，移植材料被完全替代。然而，在填充Bio-Oss Collagen的缺损处，仅观察到致密结缔组织，其中含有少量不成熟的小梁骨和大量残留的Bio-Oss颗粒。组织形态学分析显示，与Bio-Oss Collagen相比，β-TCP复合材料能引起更大的组织增生，缺损处观察到更大体积的骨组织，残留材料的生物吸收也更多。
这些结果表明，β-TCP复合材料比Bio-Oss Collagen具有更强的骨传导性和更好的生物降解特性；β-TCP-胶原蛋白复合材料的这些特性有助于骨形成和重塑。
