Deng Lei, Ai Liya, Li Runxu, Xu Wusheng, Zheng Lingling, Wang Chao, Huang Haitao
Department of Stomatology, The First Affiliated Hospital of Dalian Medical University, Dalian 116011, China.
Key Laboratory of Biomechanics and Mechanobiology, Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, School of Engineering Medicine, Beihang University, No. 37, Xueyuan Road, Beijing 100083, China.
J Funct Biomater. 2025 May 13;16(5):176. doi: 10.3390/jfb16050176.
In this study, a scaffold was designed using 3-Matic software 12.0 (Materialise, Leuven, Belgium) and fabricated via Digital Light Processing (DLP) 3D printing technology, followed by a mechanical property evaluation. The scaffold was bilaterally implanted into mandibular bone defect models in four Beagle dogs to facilitate guided alveolar bone regeneration. After 12 weeks, samples were harvested from two dogs for radiographic and histopathological evaluations. In the remaining two dogs, two dental implants were placed into the scaffold sites. After an additional 12 weeks, samples were harvested for further radiographic and histopathological assessments. (1) Compression testing of the scaffold demonstrated a compressive strength of 24.77 ± 2.36 MPa. (2) Three of the implantation sites exhibited poor wound healing and exposure of the bone grafts early post-surgery (4 weeks), with an exposure rate of 37.5%. (3) Micro-CT imaging revealed a uniform distribution of newly formed bone within the scaffold, with an average bone height of 4.05 ± 0.55 mm and a bone volume fraction of 43.93 ± 4.68%. Histopathological analysis demonstrated the presence of vascularized tissue, non-calcified bone, and newly calcified bone within the scaffold. Additionally, newly formed calcified bone and vascularized tissue were observed at the interface between the implant and the scaffold. These findings suggest that DLP 3D-printed A-W bioactive glass scaffolds represent a promising approach for guided alveolar bone regeneration in dental implant applications.
在本研究中，使用3-Matic软件12.0（Materialise，比利时鲁汶）设计了一种支架，并通过数字光处理（DLP）3D打印技术制造，随后进行力学性能评估。将该支架双侧植入4只比格犬的下颌骨缺损模型中，以促进引导性牙槽骨再生。12周后，从2只犬身上采集样本进行影像学和组织病理学评估。在其余2只犬中，在支架部位植入2颗牙种植体。再过122周周后，采集样本进行进一步的影像学和组织病理学评估。（1）支架的压缩测试显示抗压强度为24.77±2.36MPa。（2）3个植入部位在术后早期（4周）伤口愈合不良且骨移植暴露，暴露率为37.5%。（3）显微CT成像显示支架内新形成的骨分布均匀，平均骨高度为4.05±0.55mm，骨体积分数为43.93±4.68%。组织病理学分析表明支架内存在血管化组织、未钙化骨和新钙化骨。此外，在种植体与支架的界面处观察到新形成的钙化骨和血管化组织。这些发现表明，DLP 3D打印的A-W生物活性玻璃支架是牙种植应用中引导性牙槽骨再生的一种有前景的方法。
