Instituto de Biotecnologia e Bioengenharia-Centro de Biotecnologia dos Açores, Universidade dos Açores, 9701-851, Angra do Heroísmo, Portugal.
Protoplasma. 2013 Apr;250(2):495-503. doi: 10.1007/s00709-012-0432-4. Epub 2012 Jul 20.
Maize (Zea mays L.) endosperm transfer cells are essential for kernel growth and development so they have a significant impact on grain yield. Although structural and ultrastructural studies have been published, little is known about the development of these cells, and prior to this study, there was a general consensus that they contain only flange ingrowths. We characterized the development of maize endosperm transfer cells by bright field microscopy, transmission electron microscopy, and confocal laser scanning microscopy. The most basal endosperm transfer cells (MBETC) have flange and reticulate ingrowths, whereas inner transfer cells only have flange ingrowths. Reticulate and flange ingrowths are mostly formed in different locations of the MBETC as early as 5 days after pollination, and they are distinguishable from each other at all stages of development. Ingrowth structure and ultrastructure and cellulose microfibril compaction and orientation patterns are discussed during transfer cell development. This study provides important insights into how both types of ingrowths are formed in maize endosperm transfer cells.
玉米（Zea mays L.）胚乳转移细胞对于核的生长和发育至关重要，因此对谷物产量有重大影响。尽管已经发表了结构和超微结构研究，但对这些细胞的发育知之甚少，在此之前，人们普遍认为它们只含有边缘内突。我们通过明场显微镜、透射电子显微镜和共聚焦激光扫描显微镜来描述玉米胚乳转移细胞的发育过程。最基部的胚乳转移细胞（MBETC）具有边缘和网状内突，而内部转移细胞只有边缘内突。网状和边缘内突早在授粉后 5 天就在 MBETC 的不同位置形成，并且在发育的所有阶段都可以彼此区分开来。在转移细胞发育过程中讨论了内突结构和超微结构以及纤维素微纤丝的紧实和取向模式。这项研究为玉米胚乳转移细胞中这两种内突的形成方式提供了重要的见解。
