Department of Chemistry, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil.
Planta Med. 2012 Sep;78(14):1601-6. doi: 10.1055/s-0032-1315043. Epub 2012 Jul 18.
Plants are promising sources of new bioactive compounds. The aim of this study was to investigate the cytotoxic potential of nine plants found in Brazil. The species studied were: Annona pickelii Diels (Annonaceae), Annona salzmannii A. DC. (Annonaceae), Guatteria blepharophylla Mart. (Annonaceae), Guatteria hispida (R. E. Fr.) Erkens & Maas (Annonaceae), Hancornia speciosa Gomes (Apocynaceae), Jatropha curcas L. (Euphorbiaceae), Kielmeyera rugosa Choisy (Clusiaceae), Lippia gracilis Schauer (Verbenaceae), and Hyptis calida Mart. Ex Benth (Lamiaceae). Different types of extractions from several parts of plants resulted in 43 extracts. Their cytotoxicity was tested against HCT-8 (colon carcinoma), MDA-MB-435 (melanoma), SF-295 (glioblastoma), and HL-60 (promielocitic leukemia) human tumor cell lines, using the thiazolyl blue test (MTT) assay. The active extracts were those obtained from G. blepharophylla, G. hispida, J. curcas, K. rugosa, and L. gracilis. In addition, seven compounds isolated from the active extracts were tested; among them, β-pinene found in G. hispida and one coumarin isolated from K. rugora showed weak cytotoxic activity. In summary, this manuscript contributes to the understanding of the potentialities of Brazilian plants as sources of new anticancer drugs.
植物是具有生物活性的新化合物的有前途的来源。本研究的目的是研究在巴西发现的九种植物的细胞毒性潜力。研究的物种为：安诺那·皮克尔氏种（番荔枝科）、安诺那·萨尔兹曼氏种（番荔枝科）、瓜特里亚·布莱法罗菲亚种（番荔枝科）、瓜特里亚·希斯皮达种（番荔枝科）、汉科尼亚·斯佩西亚种（夹竹桃科）、麻疯树（大戟科）、基尔梅耶拉·鲁格萨种（藤黄科）、利皮亚·格雷斯利种（马鞭草科）和希普提斯·卡尔迪种（唇形科）。从植物的不同部位进行了不同类型的提取，得到了 43 种提取物。使用噻唑蓝试验（MTT）测定法，对它们的细胞毒性进行了测试，以评估它们对 HCT-8（结肠癌）、MDA-MB-435（黑色素瘤）、SF-295（胶质母细胞瘤）和 HL-60（早幼粒细胞白血病）人肿瘤细胞系的作用。活性提取物来自 G. blepharophylla、G. hispida、J. curcas、K. rugosa 和 L. gracilis。此外，还测试了从活性提取物中分离出的七种化合物；其中，在 G. hispida 中发现的β-蒎烯和从 K. rugora 中分离出的一种香豆素显示出较弱的细胞毒性活性。总之，本文有助于理解巴西植物作为新型抗癌药物来源的潜力。
