Department of Biochemistry and Molecular Biology, Monash University, Clayton Campus, Victoria, Australia.
PLoS One. 2012;7(7):e41028. doi: 10.1371/journal.pone.0041028. Epub 2012 Jul 16.
We have engineered a monomeric blue non-fluorescent chromoprotein called Ultramarine (fluorescence quantum yield, 0.001; ε(585 nm), 64,000 M(-1) x cm(-1)) for use as a Förster resonance energy transfer acceptor for a number of different donor fluorescent proteins. We show its use for monitoring activation of caspase 3 in live cells using fluorescence lifetime imaging. Ultramarine has the potential to increase the number of cellular parameters that can be imaged simultaneously.
我们设计了一种单体蓝色非荧光色蛋白，称为 Ultramarine（荧光量子产率为 0.001；ε(585nm)为 64,000 M(-1) x cm(-1)），用作许多不同供体荧光蛋白的Förster 共振能量转移受体。我们展示了它在使用荧光寿命成像监测活细胞中 caspase 3 激活方面的用途。Ultramarine 有可能增加可以同时成像的细胞参数数量。
