Mahidol-Oxford Tropical Medicine Research Unit (MORU), Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Bangkok 10400, Thailand.
Trans R Soc Trop Med Hyg. 2012 Sep;106(9):563-6. doi: 10.1016/j.trstmh.2012.06.002. Epub 2012 Jul 19.
The diagnostic utility of the Standard Diagnostics Leptospira IgM ELISA for detection of acute leptospirosis was assessed in febrile adults admitted in Vientiane, Laos. Using the cut-off suggested by the manufacturer [optical density (OD) ≥0.75], the assay demonstrated limited diagnostic capacity with a sensitivity of 95% and a specificity of 41% compared with the Leptospira microscopic agglutination test, which is the serological gold standard. However, re-evaluation of the diagnostic cut-off to an OD of 1.7 demonstrated improved diagnostic accuracy overall (sensitivity 70%; specificity 78%).
本研究旨在评估老挝万象市发热成人中应用 Standard Diagnostics Leptospira IgM ELISA 检测急性钩端螺旋体病的诊断价值。以制造商推荐的临界值(光密度(OD)≥0.75)为标准,与血清学金标准——显微镜凝集试验相比,该检测方法的灵敏度为 95%,特异度为 41%,诊断效能有限。然而,将诊断临界值重新评估为 OD 值 1.7,总体上提高了诊断准确性(灵敏度为 70%,特异度为 78%)。