Department of Biotechnology, Indian Institute of Technology, Kharagpur 721302, India.
Biomaterials. 2012 Oct;33(30):7456-67. doi: 10.1016/j.biomaterials.2012.06.091. Epub 2012 Jul 21.
In situ forming tissue sealants are advantageous due to ease in application, complete coverage of defect site and assured comfort levels to patients. The interconnected three-dimensional hydrophilic networks perfectly manage typical dermal wounds by suitably scaffolding skin fibroblast, diffusing the nutrients, therapeutics and exudates while still maintaining an adequately moist environment. We evaluate the cell homing ability of semi-interpenetrating non-mulberry tropical tasar silk sericin/polyacrylamide hydrophilic network with a keen understanding of its network characteristics and correlation of protein concentration with the performance as cell scaffold. Interconnectivity of porous networks observed through scanning electron micrograph revealed pore sizes ranging from 23 to 52 μm. The enhanced β-sheet content with the increasing sericin concentration in far red spectroscopy study supported their corresponding improved compressive strength. These semi-interpenetrating networks were found to possess a maximum fluid uptake of 112% of its weight, hence preventing the accumulation of exudates at the wound area. The present systems appear to possess characteristics like rapid gelation (~5min) at 37 °C, 98% porosity enabling the migration of fibroblasts during healing (observed through confocal and scanning electron micrographs), cell adhesion together with the absence of any cyto-toxic effect suggesting its potential as in situ tissue sealants. The compressive strength up to 61 kPa ensured ease in handling even when wet. The results prove the suitability to use non-mulberry tasar cocoon silk sericin/polyacrylamide semi-interpenetrating network as a reconstructive dermal sealant.
原位形成的组织密封剂具有应用方便、完全覆盖缺陷部位和确保患者舒适度的优点。相互连接的三维亲水网络通过适当支撑皮肤成纤维细胞，扩散营养物质、治疗剂和渗出物，同时保持足够的湿润环境，完美地管理典型的皮肤伤口。我们评估了半互穿非桑蚕热带野蚕丝素/聚丙烯酰胺亲水网络的细胞归巢能力，深入了解其网络特性以及蛋白质浓度与作为细胞支架的性能之间的相关性。通过扫描电子显微镜观察到多孔网络的连通性，发现孔径范围为 23 至 52 μm。远红光谱研究中β-折叠含量的增加随着丝素浓度的增加而增加，支持了它们相应的抗压强度的提高。这些半互穿网络被发现具有高达其重量 112%的最大流体吸收能力，从而防止渗出物在伤口区域积聚。目前的系统似乎具有在 37°C 下快速胶凝（~5 分钟）、98%的孔隙率，允许成纤维细胞在愈合过程中迁移（通过共聚焦和扫描电子显微镜观察到）、细胞黏附和不存在任何细胞毒性作用的特性，表明其作为原位组织密封剂的潜力。高达 61 kPa 的抗压强度确保了即使在潮湿时也易于处理。研究结果证明了非桑蚕野蚕丝素/聚丙烯酰胺半互穿网络作为一种重建性皮肤密封剂的适用性。
