Du Pan, Diao Ling, Lu Yichi, Liu Chenyang, Li Jin, Chen Yang, Chen Junfeng, Lv Guozhong, Chen Xue
Wuxi Medical School, Jiangnan University, Wuxi, 214122, China.
The Affifiliated Hospital of Jiangnan University, Jiangsu, 214000, China.
Heliyon. 2023 Feb 9;9(3):e13554. doi: 10.1016/j.heliyon.2023.e13554. eCollection 2023 Mar.
The treatment of full-thickness cutaneous wounds remains a significant challenge in clinical therapeutics. Exogenous growth factor (GF) has been applied in clinics to promote wound healing. However, the retention of GF on the wound bed after its direct application to the wound surface is difficult. Moreover, growth factors (GFs) are always inactivated in the complex wound healing microenvironment due to various factors, which significantly decrease the therapeutic effect. Sericin hydrogel (S) can be used as an effective carrier for GFs owing to its low immunogenicity, good biocompatibility, and good healing-promoting ability. Here, we designed a heparin-based sericin hydrogel (HS) -encapsulated basic fibroblast growth factor (bFGF-HS) to facilitate wound healing and skin regeneration. The hydrogel exhibited a three-dimensional (3D) microporous structure, excellent biodegradability, good adhesiveness, and low cytotoxicity. In vitro release of bFGF from bFGF-HS coacervates revealed that bFGF-HS might control the release of bFGF within 25 days through heparin regulation. bFGF-HS significantly promoted vascularization and re-epithelialization and improved collagen deposition, ultimately accelerating wound healing in vivo in mice. bFGF-HS treated wounds were also found to have more hair follicles and milder inflammatory reactions. Overall, this study provides a new therapeutic approach for full-thickness skin defect wounds using bFGF-HS.
全层皮肤伤口的治疗在临床治疗中仍然是一项重大挑战。外源性生长因子（GF）已应用于临床以促进伤口愈合。然而，将GF直接应用于伤口表面后，其在伤口床的保留较为困难。此外，由于各种因素，生长因子（GFs）在复杂的伤口愈合微环境中总是会失活，这显著降低了治疗效果。丝胶蛋白水凝胶（S）因其低免疫原性、良好的生物相容性和良好的促愈合能力，可作为GFs的有效载体。在此，我们设计了一种基于肝素的丝胶蛋白水凝胶（HS）包裹碱性成纤维细胞生长因子（bFGF-HS）以促进伤口愈合和皮肤再生。该水凝胶呈现三维（3D）微孔结构，具有优异的生物降解性、良好的粘附性和低细胞毒性。bFGF从bFGF-HS凝聚层的体外释放表明，bFGF-HS可能通过肝素调节在25天内控制bFGF的释放。bFGF-HS显著促进血管生成和再上皮化，并改善胶原蛋白沉积，最终加速小鼠体内伤口愈合。还发现bFGF-HS处理的伤口有更多毛囊且炎症反应较轻。总体而言，本研究为使用bFGF-HS治疗全层皮肤缺损伤口提供了一种新的治疗方法。
