Parco Tecnologico Padano, CERSA, Via Einstein, Loc. Cascina Codazza, 26900, Lodi, Italy.
Cytotechnology. 2012 Jan;64(1):1-7. doi: 10.1007/s10616-011-9398-x. Epub 2011 Oct 13.
Stem cells represent an important tool in veterinary therapeutic field such as tissue engineering. In the present study, equine amnion-derived mesenchymal stromal cells were investigated for applications in veterinary science as an alternative source to bone marrow mesenchymal stem cells and adipose stem cells. Amnion stromal cells isolation and characterization protocol is described; the in vitro cell growth rate was calculated by measuring viable cell number over 20 days. The expression of stem cell markers such as Oct-4, Nanog, Sox-2 and CD105 was assessed by retrotranscription quantitative PCR (RT-qPCR) and differentiation into adipocytes, osteocytes and chondrocytes precursors was analyzed by cytochemical staining. This study showed that amnion stromal cells expressing stem cell markers can differentiate into mesoderm lineage and may be an alternative source to mesenchymal stem cells derived from adipose tissue and bone marrow for the use in tissue repair.
干细胞是兽医治疗领域（如组织工程）的重要工具。本研究旨在探讨马胎膜来源的间充质基质细胞作为骨髓间充质干细胞和脂肪干细胞的替代物在兽医科学中的应用。本文描述了马胎膜基质细胞的分离和鉴定方案；通过在 20 天内测量活细胞数量来计算体外细胞生长速率。通过逆转录定量 PCR (RT-qPCR) 评估干细胞标志物（如 Oct-4、Nanog、Sox-2 和 CD105）的表达，并通过细胞化学染色分析其向脂肪细胞、成骨细胞和软骨细胞前体的分化。本研究表明，表达干细胞标志物的羊膜基质细胞可以分化为中胚层谱系，可能是源自脂肪组织和骨髓的间充质干细胞的替代物，可用于组织修复。
