Department of Internal Medicine I, Division of Infectious Diseases and Tropical Medicine, Medical University of Vienna, Vienna, Austria.
Eur J Immunol. 2012 Nov;42(11):2844-50. doi: 10.1002/eji.201242678. Epub 2012 Sep 20.
Mycobacterium tuberculosis (MTB)-specific cytokine responses in the peripheral blood and at the site of infection may differ significantly within the same individual, but the under-lying T-cell subset changes are largely unknown. Here, we measured effector and memory T-cell markers on CD4⁺ T cells (CD45RO, cysteine chemokine receptor (CCR)7, and CD27) in peripheral blood and at the site of active tuberculosis (TB). Additionally, T cells were stimulated overnight with purified protein derivative (PPD) and early secretory antigenic target (ESAT)-6 to determine which T-cell subset produces MTB-specific interferon (IFN)-γ. A striking decrease in CCR7 and CD27 expression on T cells was noted at the site of active TB. Likewise, IFN-γ expressing, ESAT-6 specific CD4⁺CD45RO⁺CD27⁻ T cells were dramatically increased at the site of infection but were not detectable in peripheral blood. An antigen-specific expansion of differentiated T cells at the site of active TB infection was poorly reflected in peripheral blood. Insight in these changes in MTB-specific effector T cells in different compartments of the body could lead to new approaches for immune-based diagnosis and interventions.
结核分枝杆菌（MTB）特异性细胞因子反应在同一个体的外周血和感染部位可能存在显著差异，但潜在的 T 细胞亚群变化在很大程度上尚不清楚。在这里，我们测量了外周血和活动性结核（TB）部位 CD4⁺T 细胞（CD45RO、半胱氨酸趋化因子受体（CCR）7 和 CD27）上的效应和记忆 T 细胞标志物。此外，我们用纯化蛋白衍生物（PPD）和早期分泌抗原靶（ESAT）-6 刺激 T 细胞过夜，以确定产生 MTB 特异性干扰素（IFN）-γ的 T 细胞亚群。在活动性 TB 部位，T 细胞上的 CCR7 和 CD27 表达明显下降。同样，在感染部位，表达 IFN-γ、ESAT-6 特异性的 CD4⁺CD45RO⁺CD27⁻T 细胞显著增加，但在外周血中无法检测到。在活动性 TB 感染部位，分化 T 细胞的抗原特异性扩增在外周血中反映不佳。深入了解这些 MTB 特异性效应 T 细胞在体内不同部位的变化可能为基于免疫的诊断和干预提供新的方法。
