Centre of Influenza Research & School of Public Health, University of Hong Kong.
J Infect Dis. 2012 Sep 1;206(5):640-5. doi: 10.1093/infdis/jis423. Epub 2012 Jul 20.
H5N1 influenza viruses, which cause disease in humans, have unusually high pathogenicity. The temporal response of primary human monocyte-derived macrophages infected with highly pathogenic H5N1 and seasonal H1N1 influenza viruses was evaluated using mass spectrometry-based quantitative proteomic profiling. This was done in order to demonstrate significant perturbation of the host proteome upon viral infection, as early as 1 hour after infection. This early host response distinguished H5N1 infection from H1N1 infection, the latter inducing less of a response. The most pronounced effect was observed on the translational machinery, suggesting that H5N1 might gain advantage in replication by using the cell protein synthesis machinery early in the infection.
H5N1 流感病毒可导致人类患病，其致病性异常高。本研究采用基于质谱的定量蛋白质组学分析方法，评估了高致病性 H5N1 和季节性 H1N1 流感病毒感染原代人单核细胞衍生巨噬细胞后的时间反应。其目的是为了展示病毒感染后宿主蛋白质组的显著改变，这一改变早在感染后 1 小时即可观察到。这一早期的宿主反应可将 H5N1 感染与 H1N1 感染区分开来，后者引起的反应较小。在翻译机制上观察到最显著的效应，这表明 H5N1 可能通过在感染早期利用细胞蛋白合成机制来获得复制优势。
