Institute for Medical Microbiology and Hygiene, Technical University of Dresden, Dresden, Germany.
BMC Microbiol. 2012 Jul 23;12:146. doi: 10.1186/1471-2180-12-146.
Methicillin-resistant Staphylococcus aureus (MRSA) is spreading worldwide and poses a serious public health problem, being present in hospital settings and communities. However, from the Middle East and the Arabian Peninsula few molecular typing data on MRSA strains are currently available. In order to obtain data on the population structure of MRSA in Riyadh, Saudi Arabia, 107 clinical and environmental MRSA isolates were genotyped using a microarray-based assay.
Five major MRSA strains from four clonal complexes were identified CC8/ST239-III (20.75%), PVL-positive as well as -negative CC22-IV (18.87% and 9.43%, respectively), PVL-positive CC30-IV (12.26%) and PVL-positive CC80-IV (17.92%). Minor strains, which accounted for less than 3% each, included CC1-IV/SCCfus, PVL-positive CC1/ST772-V, PVL-positive as well as- negative CC5-IV, CC5-IV/SCCfus, CC5-V, CC6-IV, CC45-IV, PVL-negative CC80-IV, PVL-positive CC88-IV, CC97-V and a CC9/ST834-MRSA strain.
Typing of MRSA strains from Riyadh revealed a high diversity of clonal complexes. The prevalence of the genes encoding the Panton-Valentine leukocidin was surprisingly high (54.21%), and a significant rate of resistance markers was detected also in strains considered as community-associated.
耐甲氧西林金黄色葡萄球菌（MRSA）在全球范围内传播，对公共卫生构成严重威胁，存在于医院环境和社区中。然而，从中东和阿拉伯半岛获得的关于 MRSA 菌株的分子分型数据很少。为了了解沙特阿拉伯利雅得地区 MRSA 的种群结构，使用基于微阵列的检测方法对 107 株临床和环境 MRSA 分离株进行了基因分型。
从四个克隆复合体中鉴定出了五种主要的 MRSA 菌株，包括 CC8/ST239-III（20.75%）、PVL 阳性和阴性 CC22-IV（分别为 18.87%和 9.43%）、PVL 阳性 CC30-IV（12.26%）和 PVL 阳性 CC80-IV（17.92%）。占比不到 3%的少数菌株包括 CC1-IV/SCCfus、PVL 阳性 CC1/ST772-V、PVL 阳性和阴性 CC5-IV、CC5-IV/SCCfus、CC5-V、CC6-IV、CC45-IV、PVL 阴性 CC80-IV、PVL 阳性 CC88-IV、CC97-V 和一株 CC9/ST834-MRSA 菌株。
对利雅得地区 MRSA 菌株的分型显示出高度的克隆复合体多样性。编码 Panton-Valentine 白细胞毒素的基因的流行率非常高（54.21%），而且在被认为是社区相关的菌株中也检测到了相当比例的耐药标记。
