Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.
Department of Biology, College of Science, University of Jeddah, Jeddah, Saudi Arabia.
Front Cell Infect Microbiol. 2024 Jan 12;13:1339339. doi: 10.3389/fcimb.2023.1339339. eCollection 2023.
is a significant human pathogen that poses a threat to public health due to its association with foodborne contamination and a variety of infections. The factors contributing to the pathogenicity of include virulence, drug resistance, and toxin production, making it essential to monitor their prevalence and genetic profiles. This study investigated and compared the genomic characteristics of isolates from retail meat and patients in Saudi Arabia.
A total of 136 isolates were obtained between October 2021 and June 2022:84 from patients and 53 from meat samples in Riyadh, Saudi Arabia. isolates were identified using conventional methods and MALDI-TOF MS, and methicillin-resistant (MRSA) was identified using VITEK2 and BD Phoenix systems. MRSA was confirmed phenotypically using chromogenic agar, and genotypically by detecting A. Genomic data were analyzed using BactopiaV2 pipeline, local BLAST, and MLST databases.
Antibiotic resistance genes were prevalent in both meat and patient isolates, with high prevalence of 38, Z, and B. Notably, all isolates from patients carried multidrug-resistant (MDR) genes, and a high percentage of isolates from meat also harbored MDR genes. Phenotypically, 43% of the isolates from meat and 100% of the patients' isolates were MDR. Enterotoxin genes, including X, , and , exhibited high compatibility between meat and patient isolates. Virulence genes such as , and were found in all isolates from both sources.
Our study established a genetic connection between isolates from meat and patients, showing shared antibiotic resistance and virulence genes. The presence of these genes in meat derived isolates underscores its role as a reservoir. Genomic relatedness also suggests potential transmission of resistance between different settings. These findings emphasize the necessity for a comprehensive approach to monitor and control infections in both animals and humans.
金黄色葡萄球菌是一种重要的人类病原体，由于其与食源性污染和多种感染有关，因此对公共健康构成威胁。金黄色葡萄球菌的致病性因素包括毒力、耐药性和毒素产生，因此监测其流行和遗传特征至关重要。本研究调查并比较了沙特阿拉伯零售肉品和患者分离株的基因组特征。
本研究于 2021 年 10 月至 2022 年 6 月期间共获得了 136 株金黄色葡萄球菌分离株：84 株来自患者，53 株来自沙特阿拉伯利雅得的肉品样本。金黄色葡萄球菌分离株采用常规方法和 MALDI-TOF MS 进行鉴定，耐甲氧西林金黄色葡萄球菌（MRSA）采用 VITEK2 和 BD Phoenix 系统进行鉴定。MRSA 采用显色琼脂进行表型确认，采用 A 基因检测进行基因型确认。基因组数据采用 BactopiaV2 管道、本地 BLAST 和 MLST 数据库进行分析。
肉品和患者金黄色葡萄球菌分离株均普遍携带抗生素耐药基因，38、Z 和 B 基因的携带率较高。值得注意的是，所有患者分离株均携带多药耐药（MDR）基因，而大部分肉品分离株也携带 MDR 基因。表型上，43%的肉品分离株和 100%的患者分离株为 MDR。肠毒素基因 X、、和 存在于肉品和患者金黄色葡萄球菌分离株之间，具有较高的相容性。所有来源的金黄色葡萄球菌分离株均携带毒力基因 、和 。
本研究建立了肉品和患者金黄色葡萄球菌分离株之间的遗传联系，表明它们具有共同的抗生素耐药和毒力基因。肉品衍生分离株中存在这些基因，突显了其作为储库的作用。基因组的亲缘关系还表明，不同环境之间可能存在耐药性的传播。这些发现强调了需要采取综合方法来监测和控制动物和人类的金黄色葡萄球菌感染。
Zotero 插件
