猿猴空泡病毒40转录复合体。II. 转录过程中蛋白质与猿猴空泡病毒40染色质的非解离
The sv40 transcription complex. II. Non-dissociation of protein from SV40 chromatin during transcription.
作者信息
Green M H, Brooks T L
出版信息
Nucleic Acids Res. 1977 Dec;4(12):4279-89. doi: 10.1093/nar/4.12.4279.
Abstract
A small fraction of the SV40 chromatin isolated from infected monkey cell cultures by the Triton method contains active RNA polymerase which had initiated transcription in vivo. This viral transcription complex (VTC) was utilized to answer the question of whether proteins dissociate from chromatin during transcription in vitro. 3H-RNA was synthesized by the VTC under conditions such that over half the label was in transcripts which were longer than half the length of the SV40 genome. Virtually all of the 3H-RNA remained associated with the SV40 chromatin, causing an increase in sedimentation rate from 55S to 78S. The density of the VTC-3H-RNA complex indicated that less than 5% of the original protein dissociated from the SV40 DNA which served as a template for transcription. We conclude that SV40 chromatin can be transcribed while the proteins remain associated with the DNA.
摘要
通过Triton法从感染的猴细胞培养物中分离出的一小部分SV40染色质含有在体内已启动转录的活性RNA聚合酶。利用这种病毒转录复合物（VTC）来回答蛋白质在体外转录过程中是否会从染色质上解离的问题。在这样的条件下，VTC合成了3H-RNA，使得超过一半的标记物存在于长度超过SV40基因组一半长度的转录本中。几乎所有的3H-RNA都与SV40染色质保持结合，导致沉降速率从55S增加到78S。VTC-3H-RNA复合物的密度表明，从作为转录模板的SV40 DNA上解离的原始蛋白质不到5%。我们得出结论，SV40染色质在蛋白质与DNA保持结合的情况下可以进行转录。
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