Division of Haematology, Immunology, Oncology, Rheumatology, and Pulmonology, Department of Internal Medicine II, University Clinic of Tübingen, Tübingen, Germany.
Adv Biochem Eng Biotechnol. 2013;129:1-17. doi: 10.1007/10_2012_147.
There is an increasing interest in adult stem cells, especially mesenchymal stem/stromal cells (MSCs), in hematology and regenerative medicine because of the simplicity of isolation and ex vivo expansion of these cells. Conventionally, MSCs are functionally isolated from tissue based on their capacity to adhere to the surface of culture flasks. This isolation procedure is hampered by the unpredictable influence of secreted molecules and interactions with co-cultured hematopoietic and other unrelated cells, as well as by the arbitrarily selected removal time of non-adherent cells prior to the expansion of MSCs. Finally, functionally isolated cells do not provide biological information about the starting population. To circumvent these limitations, several strategies have been developed to facilitate the prospective isolation of MSCs based on the selective expression or absence of surface markers. The isolation and ex vivo expansion of these cells require an adequate quality control of the source and product. Here we summarize the most frequently used markers and introduce new targets for antibody-based isolation and characterization of bone marrow-derived MSCs.
人们对成体干细胞，特别是间充质干细胞（MSCs）在血液学和再生医学中的应用越来越感兴趣，因为这些细胞的分离和体外扩增较为简单。传统上，MSCs 是根据其在培养瓶表面黏附的能力从组织中功能上分离出来的。这种分离过程受到分泌分子的不可预测影响以及与共培养的造血细胞和其他无关细胞的相互作用的阻碍，同时还受到在 MSC 扩增之前任意选择去除非黏附细胞的时间的阻碍。最后，功能上分离的细胞并不能提供关于起始群体的生物学信息。为了规避这些限制，已经开发了几种策略，以基于表面标记物的选择性表达或缺失来促进 MSCs 的预期分离。这些细胞的分离和体外扩增需要对来源和产物进行适当的质量控制。在这里，我们总结了最常用的标记物，并介绍了用于基于抗体的骨髓源性 MSCs 分离和鉴定的新靶标。
