Rowlands D J
Proc Natl Acad Sci U S A. 1979 Oct;76(10):4793-7. doi: 10.1073/pnas.76.10.4793.
The RNAs extracted from purified preparations of the Indiana and New Jersey serotypes of vesicular stomatitis virus were polyadenylylated in vitro by using polynucleotide phosphorylase and sequence determination was carried out by the dideoxynucleotide method using reverse transcriptase and dT8AC primer. On both virus RNAs a short stretch of adenylic acid residues is present between the regions coding for the leader and N protein mRNAs. Other features of the RNA sequences of the two viruses are compared to each other and to published data.
从纯化的水疱性口炎病毒印第安纳血清型和新泽西血清型制剂中提取的RNA,利用多核苷酸磷酸化酶在体外进行聚腺苷酸化,并用双脱氧核苷酸法,以逆转录酶和dT8AC引物进行序列测定。在两种病毒RNA上,编码前导mRNA和N蛋白mRNA的区域之间都存在一小段腺苷酸残基。将这两种病毒RNA序列的其他特征相互比较,并与已发表的数据进行比较。