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通过EST分析鉴定和表征食蟹猴中的微小RNA

Identification and Characterization of MicroRNAs in Macaca fascicularis by EST Analysis.

作者信息

Yang Hao, Zhang Haiyang, Zhu Lin, Zhang Chenyu, Li Donghai

机构信息

Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China.

出版信息

Comp Funct Genomics. 2012;2012:957607. doi: 10.1155/2012/957607. Epub 2012 Jul 5.

DOI:10.1155/2012/957607
PMID:22829752
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3398586/
Abstract

MicroRNAs (miRNAs) are small noncoding RNAs which repress gene expression at the posttranscriptional level. In this study, an expressed sequence tag (EST)-based combined method was applied for the detection of miRNAs in Macaca fascicularis which is used as a model animal extensively in medical experiments, particularly those involved with neuroscience and disease. Initially, previously known miRNA sequences from metazoans were used to blast with the EST databases of Macaca fascicularis, and then a range of filtering criteria was conducted to remove some pseudo ones. At last a total of 8 novel conserved miRNAs were identified; their functions were further predicted and analyzed. Together, our study provides insight into miRNAs and their functions in Macaca fascicularis, indicating that the EST analysis is an efficient and affordable alternative approach for identifying novel miRNA candidates.

摘要

微小RNA（miRNA）是一类小的非编码RNA，它们在转录后水平抑制基因表达。在本研究中，一种基于表达序列标签（EST）的组合方法被应用于食蟹猴miRNA的检测，食蟹猴在医学实验尤其是神经科学和疾病相关实验中被广泛用作模式动物。最初，使用后生动物中先前已知的miRNA序列与食蟹猴的EST数据库进行比对，然后进行一系列过滤标准以去除一些假阳性。最后共鉴定出8个新的保守miRNA；对它们的功能进行了进一步预测和分析。总之，我们的研究为食蟹猴中miRNA及其功能提供了见解，表明EST分析是鉴定新的miRNA候选物的一种有效且经济的替代方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/3398586/e9cf7fa03808/CFG2012-957607.003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/3398586/f7e2f9b8252f/CFG2012-957607.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/3398586/ffe0d030d354/CFG2012-957607.002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/3398586/e9cf7fa03808/CFG2012-957607.003a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/3398586/f7e2f9b8252f/CFG2012-957607.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/3398586/ffe0d030d354/CFG2012-957607.002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a9c/3398586/e9cf7fa03808/CFG2012-957607.003a.jpg

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引用本文的文献

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PLoS One. 2015 Nov 12;10(11):e0142708. doi: 10.1371/journal.pone.0142708. eCollection 2015.

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