Allergy and Immune Disorders, Murdoch Children's Research Institute, Melbourne Vic., Australia.
Pediatr Allergy Immunol. 2012 Nov;23(7):674-81. doi: 10.1111/j.1399-3038.2012.01328.x. Epub 2012 Jul 26.
Alterations in intestinal microflora have been linked to the development of allergic disease. Recent studies suggest that healthy infant immune development may depend on the establishment of a diverse gut microbiota rather than the presence or absence of specific microbial strains.
We investigated the relationship between diversity of gut microbiota in the early postnatal period and subsequent development of eczema and atopy in the first year of life.
Fecal samples were collected 1 wk after birth from 98 infants at high risk of allergic disease, who were followed prospectively to age 12 months. Fecal microbial diversity was assessed by terminal restriction fragment length polymorphism (T-RFLP) using restriction enzymes Sau96I and AluI, with a greater number of peaks representing greater diversity of bacterial communities.
Microbial diversity at day 7 was significantly lower in infants with eczema at age 12 months as compared to infants without eczema (AluI mean number of peaks 13.1 vs. 15.5, p = 0.003, 95% CI for difference in means -3.9, -0.8; Sau96I 14.7 vs. 17.2, p = 0.03, 95% CI -4.9, -0.3). No differences were observed for atopic compared to non-atopic infants, or infants with two allergic parents compared to those with one or no allergic parent.
A more diverse intestinal microbiota in the first week of life is associated with a reduced risk of subsequent eczema in infants at increased risk of allergic disease. Interventions that enhance microbial diversity in early life may provide an effective means for the prevention of eczema in high-risk infants.
肠道微生物群的改变与过敏性疾病的发展有关。最近的研究表明，健康婴儿的免疫发育可能依赖于多样化的肠道微生物群的建立，而不是特定微生物菌株的存在或缺失。
我们研究了生命早期肠道微生物群多样性与生命第一年发生特应性皮炎和特应性的关系。
从 98 名患有过敏疾病高风险的婴儿中收集出生后 1 周的粪便样本，前瞻性随访至 12 个月龄。使用 Sau96I 和 AluI 两种限制酶的末端限制性片段长度多态性（T-RFLP）评估粪便微生物多样性，具有更多峰代表细菌群落多样性更高。
与 12 个月龄时无特应性皮炎的婴儿相比，有特应性皮炎的婴儿在第 7 天的微生物多样性显著降低（AluI 平均峰数 13.1 比 15.5，p = 0.003，差异的 95%置信区间为-3.9，-0.8；Sau96I 14.7 比 17.2，p = 0.03，95%置信区间-4.9，-0.3）。与非特应性婴儿或有 2 位过敏父母的婴儿相比，未观察到特应性婴儿或有 1 位或无过敏父母的婴儿之间存在差异。
生命早期肠道微生物群多样性更高与过敏疾病高风险婴儿随后发生特应性皮炎的风险降低相关。增强生命早期微生物多样性的干预措施可能为高危婴儿特应性皮炎的预防提供有效手段。
