Korea Basic Science Institute, Seoul, Korea.
BMB Rep. 2012 Jul;45(7):419-24. doi: 10.5483/bmbrep.2012.45.7.248.
High-fat diets (HFD) and high-carbohydrate diets (HCD)- induced obesity through different pathways, but the metabolic differences between these diets are not fully understood. Therefore, we applied proton nuclear magnetic resonance ((1)H NMR)-based metabolomics to compare the metabolic patterns between C57BL/6 mice fed HCD and those fed HFD. Principal component analysis derived from (1)H NMR spectra of urine showed a clear separation between the HCD and HFD groups. Based on the changes in urinary metabolites, the slow rate of weight gain in mice fed the HCD related to activation of the tricarboxylic acid cycle (resulting in increased levels of citrate and succinate in HCD mice), while the HFD affected nicotinamide metabolism (increased levels of 1-methylnicotineamide, nicotinamide-N-oxide in HFD mice), which leads to systemic oxidative stress. In addition, perturbation of gut microflora metabolism was also related to different metabolic patterns of those two diets. These findings demonstrate that (1)H NMR-based metabolomics can identify diet-dependent perturbations in biological pathways.
高脂肪饮食（HFD）和高碳水化合物饮食（HCD）通过不同的途径导致肥胖，但这些饮食之间的代谢差异尚不完全清楚。因此，我们应用基于质子核磁共振（1H NMR）的代谢组学方法比较了 C57BL/6 小鼠分别喂食 HCD 和 HFD 的代谢模式。尿中 1H NMR 光谱的主成分分析显示 HCD 和 HFD 组之间有明显的分离。基于尿代谢物的变化，喂食 HCD 的小鼠体重增加缓慢与三羧酸循环的激活有关（导致 HCD 小鼠中的柠檬酸和琥珀酸水平升高），而 HFD 影响烟酰胺代谢（HFD 小鼠中的 1-甲基烟酰胺、烟酰胺-N-氧化物水平升高），导致全身性氧化应激。此外，肠道微生物群代谢的扰动也与这两种饮食的不同代谢模式有关。这些发现表明，基于 1H NMR 的代谢组学可以识别饮食依赖性的生物途径扰动。
