Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei, Taiwan.
Ultrasonics. 2013 Feb;53(2):320-7. doi: 10.1016/j.ultras.2012.06.014. Epub 2012 Jul 7.
In this study, albumin-shelled, targeted MBs (tMBs) were first demonstrated with the expectation of visualization of biodistribution of albumin-shelled tMBs. The actual biodistribution of albumin-shelled tMBs is of vital importance either for molecular imaging or for drug delivery.
Recently, albumin microbubbles (MBs) have been studied for drug and gene delivery in vitro and in vivo through cavitation. Targeted lipid-shelled MBs have been applied for ultrasound molecular imaging and conjugated with radiolabeled antibodies for whole-body biodistribution evaluations. The novelty of the work is that, in addition to the lipid tMBs, the albumin tMBs was also applied in biodistribution detection.
Multimodality albumin-shelled, (18)F-SFB-labeled VEGFR2 tMBs were synthesized, and their characteristics in mice bearing MDA-MB-231 human breast cancer were investigated with micro-positron-emission tomography (microPET) and high-frequency ultrasound (microUS).
Albumin-shelled MBs can be labeled with (18)F-SFB directly and conjugated with antibodies for dual molecular imaging. The albumin-shelled tMBs show a lifetime in 30min in the blood pool and a highly specific adherence to tumor vessels in mice bearing human breast cancer.
From the evaluations of whole-body biodistribution, the potential of the dual molecular imaging probe for drug or gene delivery in animal experiments with albumin shelled MBs has been investigated.
本研究首次制备了载白蛋白外壳的靶向 MB（tMB），以期可视化白蛋白外壳 tMB 的生物分布。白蛋白外壳 tMB 的实际生物分布对于分子成像或药物输送都至关重要。
最近，白蛋白微泡（MB）已通过空化作用在体外和体内进行了药物和基因传递的研究。靶向脂质壳 MB 已应用于超声分子成像，并与放射性标记的抗体结合用于全身生物分布评估。这项工作的新颖之处在于，除了脂质 tMB 之外，白蛋白 tMB 也应用于生物分布检测。
合成了多模态载白蛋白、（18）F-SFB 标记的 VEGFR2 tMB，并在携带 MDA-MB-231 人乳腺癌的小鼠中使用微正电子发射断层扫描（microPET）和高频超声（microUS）对其特性进行了研究。
白蛋白 MB 可以直接标记（18）F-SFB，并与抗体结合进行双重分子成像。白蛋白壳 tMB 在血液池中的半衰期为 30 分钟，在携带人乳腺癌的小鼠中与肿瘤血管具有高度特异性的结合。
从全身生物分布评估来看，用载白蛋白 MB 进行药物或基因输送的双重分子成像探针在动物实验中的潜力已得到研究。
