Ileva Lilia V, Bernardo Marcelino, Young Matthew R, Riffle Lisa A, Tatum James L, Kalen Joseph D, Choyke Peter L
Small Animal Imaging Program, Laboratory Animal Sciences Program, Leidos Biomedical Research, Frederick National Laboratory for Cancer Research, Frederick, Maryland, USA.
Molecular Imaging Program, Laboratory Animal Sciences Program, Leidos Biomedical Research, Frederick National Laboratory for Cancer Research, Frederick, Maryland, USA.
Nat Protoc. 2014 Nov;9(11):2682-92. doi: 10.1038/nprot.2014.178. Epub 2014 Oct 23.
We have developed a reliable noninvasive method for monitoring colonic tumors and mucosal inflammation in a mouse model of colon cancer using magnetic resonance colonography (MRC). After a mild cleansing enema, the colon is filled with Fluorinert, a perfluorinated liquid that does not produce a proton MR signal. The mouse is placed in a dedicated volume MR receiver coil, and high-resolution images are acquired in three planes. The Fluorinert enema distends the mouse colon, creating an artifact-free black homogeneous background, allowing clear delineation of the inflamed colonic wall and visualization of luminal tumors in various stages of development. A gadolinium-based contrast agent can be administered i.v. to the animal to detect mural inflammation or tumor vascularity. This technique is useful for serial monitoring of the effects of preventive or therapeutic strategies on tumor development without killing the animal or requiring invasive endoscopies. The animal preparation and imaging can be completed in ∼1.5 h.
我们已经开发出一种可靠的非侵入性方法，可使用磁共振结肠成像（MRC）在结肠癌小鼠模型中监测结肠肿瘤和黏膜炎症。在进行轻度清洁灌肠后，向结肠内注入全氟辛烷磺酸，这是一种不会产生质子磁共振信号的全氟化液体。将小鼠置于专用的容积磁共振接收线圈中，并在三个平面上采集高分辨率图像。全氟辛烷磺酸灌肠使小鼠结肠扩张，形成无伪影的黑色均匀背景，从而能够清晰勾勒出发炎的结肠壁，并可视化处于不同发育阶段的腔内肿瘤。可以通过静脉注射向动物施用基于钆的造影剂，以检测壁层炎症或肿瘤血管。该技术对于在不杀死动物或不需要侵入性内镜检查的情况下，连续监测预防或治疗策略对肿瘤发展的影响非常有用。动物准备和成像可以在约1.5小时内完成。
