Key Laboratory of Bioprocess of Beijing, Beijing University of Chemical Technology, Beijing 100029, China.
J Ind Microbiol Biotechnol. 2012 Nov;39(11):1725-9. doi: 10.1007/s10295-012-1171-4. Epub 2012 Jul 26.
This work describes the production of (R,R)-2,3-butanediol in Escherichia coli using glycerol by metabolic engineering approaches. The introduction of a synthetic pathway converting pyruvate to (R,R)-2,3-butanediol into wild-type E. coli strain BW25113 led to the production of (R,R)-2,3-butanediol at a titer of 3.54 g/l and a yield of 0.131 g product/g glycerol (26.7 % of theoretical maximum) with acetate (around 3.00 g/l) as the dominant by-product. We therefore evaluated the impacts of deleting the genes ackA or/and poxB that are responsible for the major by-product, acetate. This increased production of (R,R)-2,3-butanediol to 9.54 g/l with a yield of 0.333 g product/g glycerol (68.0 % of theoretical maximum) in shake flask studies. The utilization of low-priced crude glycerol to produce value-added chemicals is of great significance to the economic viability of the biodiesel industry.
本工作描述了通过代谢工程方法在大肠杆菌中利用甘油生产（R,R）-2,3-丁二醇。将丙酮酸转化为（R,R）-2,3-丁二醇的合成途径引入到野生型大肠杆菌 BW25113 菌株中，导致（R,R）-2,3-丁二醇的产量达到 3.54 g/L，产率为 0.131 g 产物/g 甘油（理论最大值的 26.7%），乙酸盐（约 3.00 g/L）为主要副产物。因此，我们评估了敲除 ackA 或/和 poxB 基因的影响，这些基因负责产生主要副产物乙酸盐。在摇瓶研究中，这将（R,R）-2,3-丁二醇的产量提高到 9.54 g/L，产率为 0.333 g 产物/g 甘油（理论最大值的 68.0%）。利用廉价的粗甘油生产高附加值化学品对生物柴油工业的经济可行性具有重要意义。
