Choi O K, Song Y S
Bioanalytical Laboratory, Dongyoung Instruments, Seoul, South Korea.
J Pharm Biomed Anal. 1997 Jun;15(9-10):1265-70. doi: 10.1016/s0731-7085(96)02047-x.
Effective monitoring of cefuroxim levels by micellar electrokinetic capillary chromatography with direct serum injection are discussed and compared with the HPLC method. With capillary electrophoresis (CE), in contrast to HPLC, good resolution and efficiency was demonstrated as well as low consumption of solvent and samples. The CE system was applied at 15 kV with UV detection at 274 nm using 150 mM sodium dodecyl sulfate in 20 mM sodium phosphate and borate (pH 9.0) as electrolyte. The results were seen within 12 min with efficiency approaching 182,000 theoretical plates. The coefficients of variations of migration time and peak area were less than 0.8 and 5.9%, respectively. The detection limits for quantitative determination were 0.28 microM level. Good linearity and recovery were also obtained in the range of serum levels usually encountered in clinical analysis with a correlation coefficient of r = 0.991 and 98-101% recovery. The monitoring of cefuroxim in human serum with micellar electrokinetic capillary chromatography (MECC) is demonstrated. Identification of cefuroxim in human serum with MECC is demonstrated. Identification of cefuroxim was performed by characterizing the sample peak in terms of the migration time and UV spectrum. Considering the results of our study, the CE method should by highly suitable for the separation of cefuroxim in biofluids.
讨论了通过直接进样血清的胶束电动毛细管色谱法有效监测头孢呋辛水平，并与高效液相色谱法进行了比较。与高效液相色谱法相比，毛细管电泳（CE）具有良好的分离度和效率，同时溶剂和样品消耗低。CE系统在15 kV电压下运行，以20 mM磷酸钠和硼酸盐（pH 9.0）中的150 mM十二烷基硫酸钠为电解质，在274 nm处进行紫外检测。12分钟内即可得到结果，柱效接近182,000理论塔板数。迁移时间和峰面积的变异系数分别小于0.8%和5.9%。定量测定的检测限为0.28 microM水平。在临床分析中通常遇到的血清水平范围内也获得了良好的线性和回收率，相关系数r = 0.991，回收率为98 - 101%。证明了用胶束电动毛细管色谱法（MECC）监测人血清中的头孢呋辛。证明了用MECC鉴定人血清中的头孢呋辛。通过根据迁移时间和紫外光谱表征样品峰来鉴定头孢呋辛。考虑到我们的研究结果，CE方法非常适合于生物流体中头孢呋辛的分离。
