Department of Chemistry, Yale University, New Haven, Connecticut 06520-8107, USA.
J Am Chem Soc. 2012 Aug 15;134(32):13208-11. doi: 10.1021/ja305360q. Epub 2012 Aug 6.
We report here a novel noncovalent synthetic strategy for template-assembled de novo protein design. In this approach, a peptide was first conjugated with two oligoguanosine strands via click chemistry and the conjugates were then self-assembled in the presence of metal ions. G-quadruplex formation directs two peptide strands to assemble on one face of the scaffold and form an adjacent two loop surface. This approach can be used to rapidly prepare multiple two-loop structures with both homo- and heterosequences.
我们在此报告了一种新颖的非共价合成策略，用于模板组装的从头设计蛋白质。在这种方法中，首先通过点击化学将肽与两条寡聚鸟苷链连接，然后在金属离子存在下自组装。G-四链体的形成指导两条肽链在支架的一个面上组装，并形成相邻的两个环表面。这种方法可以快速制备具有同序列和异序列的多个两个环结构。
