School of Molecular Sciences, Arizona State University, Tempe, AZ 85287, USA.
Center for Molecular Design and Biomimetics, The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.
Chem Commun (Camb). 2022 Mar 24;58(25):4044-4047. doi: 10.1039/d1cc04970d.
We report a proximity-enhanced method to synthesize a peptide flanked by two different oligonucleotide handles. Our method relies on sequential bioorthogonal reactions, and partial hybridization of the second handle to the first. We demonstrate the synthesis of a protease-responsive DNA "latch" and a cyclic bioactive peptide using this method.
我们报告了一种通过邻近增强方法合成由两个不同寡核苷酸接头侧翼的肽的方法。我们的方法依赖于顺序的生物正交反应，以及第二个接头与第一个接头的部分杂交。我们使用这种方法证明了蛋白酶响应 DNA“闩锁”和环状生物活性肽的合成。
