Schneider W, Wahl M, Kuschinsky W, Thurau K
Pflugers Arch. 1977 Nov 25;372(1):103-7. doi: 10.1007/BF00582214.
pH microelectrodes with pointed tip (Hinke-type) were constructed for the continuous measurement of the local pH in the perivascular space of pial arteries in the feline cerebral cortex. The sensitive tip had a length of 20-60 mu and a base diameter of 10-25 mu. As reference electrode, a micropipette (tip diameter 2 mu), filled with 150 mM KCl was used. Calibration curves were linear and showed a sensitivity of 54.5-57.5 mV/pH unit at 38 degrees C. Advantages of such electrodes are the easy penetration of the subarachnoid membrane, the long life span, the quick response, and a minimal drift. The electrodes were tested in vivo during hyper- and hypoventilation and during local perivascular injection of mock spinal fluid at varying pH. A close correlation was observed between the change in perivascular pH and the corresponding change in pial arterial diameter.
构建了具有尖状尖端的pH微电极（欣克型），用于连续测量猫脑皮质软脑膜动脉血管周围间隙的局部pH值。敏感尖端长度为20 - 60微米，基部直径为10 - 25微米。作为参比电极，使用了填充150 mM氯化钾的微量移液器（尖端直径2微米）。校准曲线呈线性，在38℃时显示出54.5 - 57.5 mV/pH单位的灵敏度。这种电极的优点是易于穿透蛛网膜、寿命长、响应快且漂移极小。在体内对电极进行了测试，测试过程包括过度通气和通气不足，以及在不同pH值下局部血管周围注射模拟脑脊液。观察到血管周围pH值的变化与软脑膜动脉直径的相应变化之间存在密切相关性。
