Research Laboratory of Trace elements, free radicals and antioxidants, Biophysical Department, Faculty of Medicine, University of Monastir, 5000 Monastir, Tunisia.
Prog Neuropsychopharmacol Biol Psychiatry. 2012 Dec 3;39(2):371-5. doi: 10.1016/j.pnpbp.2012.07.013. Epub 2012 Jul 27.
Numerous evidence and proofs suggest that the oxidative stress contributes to the pathogenesis of schizophrenia (SZ) and bipolar disorder (BD). The aim of this study is to determine the glutathione levels and the antioxidant enzyme activities in blood samples of patients suffering from SZ and patients with bipolar disorder in comparison with the healthy controlled subjects. It was a case-controlled study carried on upon three groups: forty-six SZ patients (41 men and 5 women, mean age=33.2±7years), thirty BD patients (25 men and 5 women, mean age=31.3±8years) and forty healthy controls (33 men and 7 women, mean age=32.3±7years). The glutathione levels are the total glutathione (GSHt), the reduced glutathione (GSHr), and the oxidized glutathione (GSSG) and the antioxidant enzyme activities that are the superoxide dismutase (SOD), the glutathione peroxidase (GPx), and the catalase (CAT) are determined by the spectrophotometer. We noticed that the GSHt and the GSHr levels significantly decreased in both SZ and BD patients in comparison with the healthy control subjects. As for SOD and CAT activities they remained lower for the patients with SZ when compared both with the controls or the BD patients. We noticed as well that the CAT activity was significantly lower in the BD group than that in the control group, whereas, GPx activity showed no significant change in each group. Hence, this report of the decreased plasma levels of GSHt and GSHr, and the impaired antioxidant enzyme activities in SZ and BD patients aims at highlighting the GSH deficit that seems to be contributing to these disorders, and showing that it may be an important indirect biomarker of the oxidative stress for the SZ and BD.
大量证据表明，氧化应激与精神分裂症（SZ）和双相情感障碍（BD）的发病机制有关。本研究旨在确定患有 SZ 和双相情感障碍的患者与健康对照者的血液样本中的谷胱甘肽水平和抗氧化酶活性。这是一项病例对照研究，共涉及三组人群：46 名 SZ 患者（41 名男性和 5 名女性，平均年龄=33.2±7 岁）、30 名 BD 患者（25 名男性和 5 名女性，平均年龄=31.3±8 岁）和 40 名健康对照者（33 名男性和 7 名女性，平均年龄=32.3±7 岁）。谷胱甘肽水平包括总谷胱甘肽（GSHt）、还原型谷胱甘肽（GSHr）和氧化型谷胱甘肽（GSSG），抗氧化酶活性包括超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（CAT），均通过分光光度计测定。我们注意到，与健康对照组相比，SZ 和 BD 患者的 GSHt 和 GSHr 水平显著降低。SOD 和 CAT 活性在 SZ 患者中均低于对照组和 BD 患者。我们还注意到，BD 组的 CAT 活性明显低于对照组，而各组的 GPx 活性均无明显变化。因此，本研究报告 SZ 和 BD 患者的血浆 GSHt 和 GSHr 水平降低，抗氧化酶活性受损，旨在强调 GSH 缺乏可能与这些疾病有关，并表明其可能是 SZ 和 BD 氧化应激的一个重要间接生物标志物。
