Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
Biochem Biophys Res Commun. 2012 Aug 24;425(2):134-7. doi: 10.1016/j.bbrc.2012.07.040. Epub 2012 Jul 24.
We have determined the spatial arrangement of rhodopsin in the retinal rod outer segment (ROS) membrane by measuring the distances between rhodopsin molecules in which native cysteines were spin-labeled at ~1.0 mol/mol rhodopsin. The echo modulation decay of pulsed electron double resonance (PELDOR) from spin-labeled ROS curved slightly with strong background decay. This indicated that the rhodopsin was densely packed in the retina and that the rhodopsin molecules were not aligned well. The curve was simulated by a model in which rhodopsin is distributed randomly as monomers in a planar membrane.
我们通过测量以 ~1.0 mol/mol 视紫红质的天然半胱氨酸进行自旋标记的视紫红质分子之间的距离，确定了视紫红质在视网膜杆状外节（ROS）膜中的空间排列。来自自旋标记 ROS 的脉冲电子双共振（PELDOR）的回波调制衰减随强背景衰减而略有变化。这表明视紫红质在视网膜中紧密堆积，并且视紫红质分子没有很好地对齐。该曲线通过视紫红质作为单体随机分布在平面膜中的模型进行模拟。
