Gomes L M C, Gesteira A S, de Almeida A-A F, de Castro A V, Dias L O, Pirovani C P, Gomes F P
Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil.
Genet Mol Res. 2012 Aug 16;11(3):2694-707. doi: 10.4238/2012.June.27.4.
Sibipiruna (Caesalpinia peltophoroides Benth) is a tree of the Brazilian Atlantic Forest. It is a flowering ornamental tree widely planted throughout Brazil and indicated for restoration of degraded areas. We examined protein profile changes in leaves of seedlings of C. peltophoroides grown in nutrient solution under greenhouse conditions, after exposure to cadmium (Cd; 32 mg/L). A two-dimensional gel was used to analyze proteins expressed in response to stress 24 and 72 h after initiation of treatment with Cd. Various protein bands were identified that were related to stress response and/or metabolic adjustments, including proteins involved with resistance to stress, including detoxification, degradation, antioxidant, transport, signal transduction, photosynthesis, electron transport, biosynthesis reactions, and transcription regulation. After 24 h of Cd exposure, the genes of most of these proteins were upregulated. These putative proteins were associated with resistance to stress, including heat shock proteins, heat stress transcriptional factor and other transcriptional factors, aquaporins, glutathione transferase and choline monooxygenase. Most of the putative proteins observed after 72 h of exposure to Cd were downregulated. They were mainly photosynthetic process proteins, such as NAD(P)H-quinone oxidoreductase, photosystem I assembly, and photosystem II CP47 chlorophyll apoprotein. There were also proteins involved with degradation, biosynthesis and antioxidant activity, such as ATP-dependent Clp protease, methylthioribose-1-phosphate and glutathione peroxidase 2. Based on preliminary proteomic analysis, we conclude that proteins related to photosynthetic activity are inhibited, decreasing plant performance under stress conditions and that several proteins related to defense mechanisms are activated, inducing the plant defense response.
西比皮鲁纳（Caesalpinia peltophoroides Benth）是一种生长在巴西大西洋森林的树木。它是一种开花观赏树，在巴西各地广泛种植，可用于退化地区的恢复。我们研究了温室条件下在营养液中生长的西比皮鲁纳幼苗叶片在暴露于镉（Cd；32 mg/L）后的蛋白质谱变化。使用二维凝胶分析在Cd处理开始后24小时和72小时响应胁迫表达的蛋白质。鉴定出了各种与胁迫反应和/或代谢调节相关的蛋白带，包括参与胁迫抗性的蛋白质，包括解毒、降解、抗氧化、转运、信号转导、光合作用、电子传递、生物合成反应和转录调控。在Cd暴露24小时后，这些蛋白质中的大多数基因上调。这些推定的蛋白质与胁迫抗性相关，包括热休克蛋白、热胁迫转录因子和其他转录因子、水通道蛋白、谷胱甘肽转移酶和胆碱单加氧酶。在暴露于Cd 72小时后观察到的大多数推定蛋白质下调。它们主要是光合过程蛋白质，如NAD(P)H-醌氧化还原酶、光系统I组装和光系统II CP47叶绿素载脂蛋白。也有参与降解、生物合成和抗氧化活性的蛋白质，如ATP依赖性Clp蛋白酶、甲基硫代核糖-1-磷酸和谷胱甘肽过氧化物酶2。基于初步的蛋白质组学分析，我们得出结论，与光合活性相关的蛋白质受到抑制，在胁迫条件下植物性能下降，并且几种与防御机制相关的蛋白质被激活，诱导植物防御反应。
