Pattathil Sivakumar, Avci Utku, Miller Jeffrey S, Hahn Michael G
BioEnergy Science Center, Complex Carbohydrate Research Center, The University of Georgia, Athens, GA, USA.
Methods Mol Biol. 2012;908:61-72. doi: 10.1007/978-1-61779-956-3_6.
The native complexity of plant cell walls makes research on them challenging. Hence, it is advantageous to have a diversity of tools that can be used to analyze and characterize plant cell walls. In this chapter, we describe one of two immunological approaches that can be employed for screening of plant cell wall/biomass materials from diverse plants and tissues. This approach, Glycome Profiling, lends itself well to moderate to high-throughput screening of plant cell wall/biomass samples. Glycome Profiling is being further optimized to reduce the amount of sample required for the analysis, and to improve the sensitivity and throughput of the assay. We are optimistic that Glycome Profiling will prove to be a broadly applicable experimental approach that will find increasing application to a wide variety of studies on plant cell wall/biomass samples.
植物细胞壁固有的复杂性使得对其进行研究具有挑战性。因此，拥有多种可用于分析和表征植物细胞壁的工具是很有优势的。在本章中，我们描述了两种免疫学方法中的一种，该方法可用于筛选来自不同植物和组织的植物细胞壁/生物质材料。这种方法，即糖组分析，非常适合对植物细胞壁/生物质样品进行中到高通量的筛选。糖组分析正在进一步优化，以减少分析所需的样品量，并提高检测的灵敏度和通量。我们乐观地认为，糖组分析将被证明是一种广泛适用的实验方法，将越来越多地应用于对各种植物细胞壁/生物质样品的研究。
