Avci Utku, Pattathil Sivakumar, Hahn Michael G
BioEnergy Science Center Complex Carbohydrate Research Center, The University of Georgia, Athens, GA, USA.
Methods Mol Biol. 2012;908:73-82. doi: 10.1007/978-1-61779-956-3_7.
Plant cell walls are dynamic structures that show changes in composition and configuration depending on the developmental stage, biotic, and abiotic factors. Therefore, it is necessary to have tools for visualizing the components of the cell wall in situ at any stage. Here, we describe how specific monoclonal antibodies can be used to examine the distribution of plant cell wall glycan epitopes at the whole plant, tissue, cell, and subcellular levels. Understanding the basic cell wall structure is essential for devising efficient strategies to convert cell walls to fermentable sugars for ethanol production.
植物细胞壁是动态结构，其组成和构型会根据发育阶段、生物和非生物因素而发生变化。因此，有必要具备在任何阶段对细胞壁成分进行原位可视化的工具。在此，我们描述了如何使用特定的单克隆抗体在全植株、组织、细胞和亚细胞水平上检测植物细胞壁聚糖表位的分布。了解基本的细胞壁结构对于设计将细胞壁转化为可发酵糖以生产乙醇的有效策略至关重要。
