Kračun Stjepan Krešimir, Fangel Jonatan Ulrik, Rydahl Maja Gro, Pedersen Henriette Lodberg, Vidal-Melgosa Silvia, Willats William George Tycho
Section for Plant Glycobiology, Department for Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark.
Methods Mol Biol. 2017;1503:147-165. doi: 10.1007/978-1-4939-6493-2_12.
Cell walls are an important feature of plant cells and a major component of the plant glycome. They have both structural and physiological functions and are critical for plant growth and development. The diversity and complexity of these structures demand advanced high-throughput techniques to answer questions about their structure, functions and roles in both fundamental and applied scientific fields. Microarray technology provides both the high-throughput and the feasibility aspects required to meet that demand. In this chapter, some of the most recent microarray-based techniques relating to plant cell walls are described together with an overview of related contemporary techniques applied to carbohydrate microarrays and their general potential in glycoscience. A detailed experimental procedure for high-throughput mapping of plant cell wall glycans using the comprehensive microarray polymer profiling (CoMPP) technique is included in the chapter and provides a good example of both the robust and high-throughput nature of microarrays as well as their applicability to plant glycomics.
细胞壁是植物细胞的一个重要特征，也是植物糖组的主要组成部分。它们具有结构和生理功能，对植物的生长发育至关重要。这些结构的多样性和复杂性需要先进的高通量技术来解答关于其结构、功能以及在基础科学和应用科学领域中作用的问题。微阵列技术提供了满足这一需求所需的高通量和可行性。在本章中，将介绍一些与植物细胞壁相关的最新基于微阵列的技术，并概述应用于碳水化合物微阵列的相关当代技术及其在糖科学中的一般潜力。本章还包括使用综合微阵列聚合物分析（CoMPP）技术对植物细胞壁聚糖进行高通量绘图的详细实验步骤，该步骤很好地展示了微阵列强大的高通量特性及其在植物糖组学中的适用性。
