苯并卟啉衍生物的血浆分布及其血浆脂蛋白对其生物分布的影响。

The plasma distribution of benzoporphyrin derivative and the effects of plasma lipoproteins on its biodistribution.

作者信息

Allison B A, Pritchard P H, Richter A M, Levy J G

机构信息

Department of Microbiology, University of British Columbia, Vancouver, Canada.

出版信息

Photochem Photobiol. 1990 Sep;52(3):501-7. doi: 10.1111/j.1751-1097.1990.tb01792.x.

DOI:10.1111/j.1751-1097.1990.tb01792.x

PMID:2284344

Abstract

The plasma distribution and biodistribution of benzoporphyrin derivative were examined. Two analogs of benzoporphyrin derivative were mixed with human plasma in vitro and recovered in the lipoprotein fractions upon separation by chromatography or ultracentrifugation. The majority of both analogs was recovered with high density lipoprotein. The effect of prebinding benzoporphyrin derivative to lipoproteins on the biodistribution of the drug in vivo was studied in tumor bearing DBA/2J mice. At 3, 8 and 24 h post-injection, tumor and tissue samples were excised and analyzed for benzoporphyrin derivative content. Precomplexing benzoporphyrin derivative with low density lipoprotein or high density lipoprotein led to significantly (P less than 0.05) greater tumor accumulation than in aqueous solution.

摘要

研究了苯并卟啉衍生物的血浆分布和生物分布。将两种苯并卟啉衍生物类似物与人体血浆在体外混合，通过色谱法或超速离心分离后，在脂蛋白组分中回收。两种类似物的大部分都与高密度脂蛋白一起回收。在荷瘤DBA/2J小鼠中研究了苯并卟啉衍生物与脂蛋白预结合对该药物体内生物分布的影响。在注射后3、8和24小时，切除肿瘤和组织样本并分析苯并卟啉衍生物含量。苯并卟啉衍生物与低密度脂蛋白或高密度脂蛋白预先复合后，肿瘤蓄积量比在水溶液中显著（P小于0.05）增加。