Netto M M, Fodor S P, Mathies R A
Department of Chemistry, University of California, Berkeley 94720.
Photochem Photobiol. 1990 Sep;52(3):605-7. doi: 10.1111/j.1751-1097.1990.tb01806.x.
Ultraviolet resonance Raman spectra of bacteriorhodopsin have been obtained using 229 nm excitation from a hydrogen-shifted neodymium yttrium aluminum garnet (Nd: YAG) laser. High signal-to-noise spectra are observed exhibiting vibrational bands at 762, 877, 1011, 1175, 1356, 1552 and 1617 cm-1 which are assigned to scattering from tryptophan and tyrosine side chains. This demonstrates the feasibility of using UV resonance Raman spectroscopy to monitor aromatic amino acid structural changes during the bacteriorhodopsin photocycle.
利用氢频移钕钇铝石榴石（Nd:YAG）激光器发出的229nm激发光，获得了细菌视紫红质的紫外共振拉曼光谱。观察到高信噪比的光谱，其在762、877、1011、1175、1356、1552和1617cm-1处呈现振动带，这些振动带归因于色氨酸和酪氨酸侧链的散射。这证明了使用紫外共振拉曼光谱监测细菌视紫红质光循环过程中芳香族氨基酸结构变化的可行性。
