Koncicki A, Tykałowski B, Stenzel T, Smiałek M, Pestka D
Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-718 Olsztyn, Poland.
Pol J Vet Sci. 2012;15(2):215-20. doi: 10.2478/v10181-011-0136-2.
The aim of the study was to determine the effect of a Polish low-virulence isolate of haemorrhagic enteritis adenovirus (HEV) on the immune system in turkeys and on the course of colibacillosis in birds infected under laboratory conditions. Turkeys were infected per os with HEV at the dose of 10(4.3)EID50/mL and with E. coli (APEC) (serotypes 078:K80:H9) at the dose of 4x10(9)CFU/mL by injection to the thoracic air sac. The birds infected with the HEV were infected with the APEC either simultaneously or after 5 days. Five days after HEV infection, the percentages of subpopulations of the CD3+CD4+ and CD3+CD8alpha+ T cells and the IgM+ B cells were determined in blood and spleens of the HEV-infected turkeys and in the control (uninfected) birds. The course of colibacillosis was more severe in turkeys infected with the APEC 5 days after infection with the HEV than in those infected with the HEV and APEC simultaneously and than in those infected only with APEC. Five turkeys out of the 18 infected with the APEC 5 days after infection with HEV, died. Their body weights were statistically significantly lower with higher FCR values 41 days after the infection in comparison to turkeys in the other groups. A considerable decrease in the percentage of the T and B cells subpopulations in the blood were found in turkeys infected with the HEV and while the percentage of CD3+CD4+ T cells subpopulation in the spleen increased significantly, the contribution of the CD3+CD8alpha+ T cells and IgM+ B cells subpopulations were decreased. These changes in the immune system of turkeys, occurring 5 days after infection with the HEV, made them more susceptible to infection with the APEC.
本研究的目的是确定波兰低毒力出血性肠炎腺病毒（HEV）分离株对火鸡免疫系统以及在实验室条件下感染禽类的大肠杆菌病病程的影响。通过经口以10(4.3)EID50/mL的剂量感染HEV，通过向胸气囊注射以4x10(9)CFU/mL的剂量感染大肠杆菌（APEC，血清型078:K80:H9）。感染HEV的禽类同时或在5天后感染APEC。在HEV感染火鸡以及对照（未感染）禽类的血液和脾脏中，于HEV感染5天后测定CD3+CD4+和CD3+CD8alpha+ T细胞亚群以及IgM+ B细胞的百分比。与同时感染HEV和APEC以及仅感染APEC的禽类相比，在感染HEV 5天后感染APEC的火鸡中，大肠杆菌病的病程更为严重。在感染HEV 5天后感染APEC的18只火鸡中有5只死亡。与其他组的火鸡相比，感染41天后，它们的体重在统计学上显著更低，饲料转化率更高。在感染HEV的火鸡中发现血液中T和B细胞亚群的百分比显著下降，而脾脏中CD3+CD4+ T细胞亚群的百分比显著增加，CD3+CD8alpha+ T细胞和IgM+ B细胞亚群的占比下降。在感染HEV 5天后火鸡免疫系统发生的这些变化，使它们更容易感染APEC。
