Department of Dermatology, Faculty of Medicine, Celal Bayar University, Manisa, Turkey.
Dis Markers. 2012;33(2):85-9. doi: 10.3233/DMA-2012-0908.
The aim of this study was to determine serum vitamin B12, folic acid and homocysteine (Hcy) levels as well as MTHFR (C677, A1298C) gene polymorphisms in patients with vitiligo, and to compare the results with healthy controls. Forty patients with vitiligo and 40 age and sex matched healthy subjects were studied. Serum vitamin B12 and folate levels were determined by enzyme-linked immunosorbent assay. Plasma Hcy levels and MTHFR polymorphisms were determined by chemiluminescence and real time PCR methods, respectively. Mean serum vitamin B12 and Hcy levels were not significantly different while folic acid levels were significantly lower in the control group. There was no significant relationship between disease activity and vitamin B12, folic acid and homocystein levels. No significant difference in C677T gene polymorphism was detected. Heterozygote A1298C gene polymorphism in the patient group was statistically higher than the control group. There was no significant relationship between MTHFR gene polymorphisms and vitamin B12, folic acid and homocysteine levels. In conclusion, vitamin B12, folate and Hcy levels are not altered in vitiligo and MTHFR gene mutations (C677T and A1298C) do not seem to create susceptibility for vitiligo.
本研究旨在测定白癜风患者血清维生素 B12、叶酸和同型半胱氨酸（Hcy）水平以及 MTHFR（C677、A1298C）基因多态性，并与健康对照组进行比较。研究纳入了 40 例白癜风患者和 40 名年龄和性别匹配的健康对照者。采用酶联免疫吸附试验测定血清维生素 B12 和叶酸水平，采用化学发光和实时 PCR 方法分别测定血浆 Hcy 水平和 MTHFR 多态性。结果显示，对照组血清维生素 B12 和 Hcy 水平无显著差异，而叶酸水平显著降低。疾病活动度与维生素 B12、叶酸和同型半胱氨酸水平之间无显著相关性。未检测到 C677T 基因多态性的显著差异。患者组杂合子 A1298C 基因多态性显著高于对照组。MTHFR 基因多态性与维生素 B12、叶酸和同型半胱氨酸水平之间无显著相关性。总之，白癜风患者维生素 B12、叶酸和 Hcy 水平并未改变，MTHFR 基因突变（C677T 和 A1298C）似乎不会导致白癜风易感性。
