Natural Products Research Institute, Gyeonggi Institute of Science and Technology Promotion, 864-1 Ieui-dong, Yeoungtong-gu, Suwon-si, Gyeonggi-do, 443-766, Republic of Korea.
Phytother Res. 2013 May;27(5):761-6. doi: 10.1002/ptr.4779. Epub 2012 Jul 31.
Adenophora triphylla var. japonica (Campanulaceae) is known to have anti-inflammatory and anti-tussive effects. Dysfunction of adipocytes and adipose tissue in obesity is related to various inflammatory cytokines or adipokines. In this study, we investigated whether lupenone isolated from A. triphylla var. japonica extract inhibits adipocyte differentiation and expression of adipogenic marker genes in 3T3-L1 preadipocytes. We demonstrated that lupenone resulted in a significant reduction in lipid accumulation and expression of adipogenic marker genes in a dose-dependent manner. In addition, lupenone decreased the transcriptional activity of peroxisome proliferator-activated receptor γ (PPARγ) induced by troglitazone, and we also demonstrated that lupenone suppressed the PPARγ and CCAAT-enhancer-binding protein α (C/EBPα) protein levels. These findings demonstrated that lupenone isolated from A. triphylla var. japonica extract effectively inhibited adipocyte differentiation through downregulation of related transcription factor, particularly the PPARγ gene.
三叶菜（桔梗科）具有抗炎和镇咳作用。肥胖症中脂肪细胞和脂肪组织的功能障碍与各种炎症细胞因子或脂肪因子有关。在这项研究中，我们研究了从三叶菜（桔梗科）提取物中分离出的羽扇豆酮是否抑制 3T3-L1 前体脂肪细胞的脂肪细胞分化和脂肪生成标记基因的表达。结果表明，羽扇豆酮可显著降低脂肪细胞中脂质的积累和脂肪生成标记基因的表达，呈剂量依赖性。此外，羽扇豆酮降低了曲格列酮诱导的过氧化物酶体增殖物激活受体γ（PPARγ）的转录活性，并且我们还证明了羽扇豆酮抑制了 PPARγ 和 CCAAT 增强子结合蛋白α（C/EBPα）蛋白水平。这些发现表明，从三叶菜（桔梗科）提取物中分离出的羽扇豆酮通过下调相关转录因子，特别是 PPARγ 基因，有效抑制脂肪细胞分化。
