Lakthan Thitiporn, Limpachayaporn Panupun, Rayanil Kanok-On, Charoenpanich Pornsri, Phuangbubpha Pornwipa, Charoenpanich Adisri
Department of Biology, Faculty of Science, Silpakorn University, Nakhon Pathom 73000, Thailand.
Department of Chemistry, Faculty of Science, Silpakorn University, Nakhon Pathom 73000, Thailand.
Life (Basel). 2023 Aug 11;13(8):1724. doi: 10.3390/life13081724.
L. (CQ) has potential as a therapeutic for managing obesity and balancing metabolic activity, but the main bioactive compound and regulatory mechanism remain unknown. Herein, the CQ hexane extract was fractionated into 30 fractions (CQ-H) using flash column chromatography and analyzed using thin-layer chromatography. The direct antiadipogenesis effect of CQ-H fractions was tested on 3T3-L1 preadipocytes. Lupenone-rich fractions 2H and 3H were identified as containing potent antiadipogenesis agents that reduced differentiated cell numbers and intracellular lipid droplet size. Although the overall mitochondrial density remained unchanged, differentiated cells exhibited a higher mitochondrial density than that in non-differentiated cells. Additionally, 2H increased mitochondrial activity in both cell types as shown by their differentiation and lipid formation stages. Lupenone was isolated from 2H (Lu-CQ) and shown to dose-dependently inhibit adipogenesis, with 2H being more potent than Lu-CQ. Lu-CQ and 2H downregulated the expression of mRNA and upregulated that of glucose transporter genes, and . Lu-CQ and 2H induced increased glucose uptake by 3T3-L1 cells. These findings suggest that lupenone-rich fractions in CQ contribute to balancing metabolic activity and reducing adipose tissue formation. Further exploration of CQ and its components may prompt innovative strategies for managing obesity and metabolic disorders.
刺蒺藜（CQ）具有作为治疗肥胖和平衡代谢活动的药物的潜力，但其主要生物活性化合物和调节机制仍不清楚。在此，使用快速柱色谱法将CQ己烷提取物分离为30个馏分（CQ-H），并使用薄层色谱法进行分析。在3T3-L1前脂肪细胞上测试了CQ-H馏分的直接抗脂肪生成作用。富含羽扇豆酮的馏分2H和3H被鉴定为含有有效的抗脂肪生成剂，可减少分化细胞数量和细胞内脂滴大小。尽管总体线粒体密度保持不变，但分化细胞的线粒体密度高于未分化细胞。此外，如在其分化和脂质形成阶段所示，2H在两种细胞类型中均增加了线粒体活性。从2H中分离出羽扇豆酮（Lu-CQ），并显示其剂量依赖性地抑制脂肪生成，2H比Lu-CQ更有效。Lu-CQ和2H下调了mRNA的表达，并上调了葡萄糖转运蛋白基因、和的表达。Lu-CQ和2H诱导3T3-L1细胞的葡萄糖摄取增加。这些发现表明，CQ中富含羽扇豆酮的馏分有助于平衡代谢活动并减少脂肪组织形成。对CQ及其成分的进一步探索可能会催生出管理肥胖和代谢紊乱的创新策略。
