National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, Sichuan, People's Republic of China.
Int J Nanomedicine. 2012;7:3365-78. doi: 10.2147/IJN.S32264. Epub 2012 Jul 4.
In recent years, interest in magnetic biomimetic scaffolds for tissue engineering has increased considerably. A type of magnetic scaffold composed of magnetic nanoparticles (MNPs) and hydroxyapatite (HA) for bone repair has been developed by our research group.
In this study, to investigate the influence of the MNP content (in the scaffolds) on the cell behaviors and the interactions between the magnetic scaffold and the exterior magnetic field, a series of MNP-HA magnetic scaffolds with different MNP contents (from 0.2% to 2%) were fabricated by immersing HA scaffold into MNP colloid. ROS 17/2.8 and MC3T3-E1 cells were cultured on the scaffolds in vitro, with and without an exterior magnetic field, respectively. The cell adhesion, proliferation and differentiation were evaluated via scanning electron microscopy; confocal laser scanning microscopy; and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), alkaline phosphatase, and bone gla protein activity tests.
The results demonstrated the positive influence of the magnetic scaffolds on cell adhesion, proliferation, and differentiation. Further, a higher amount of MNPs on the magnetic scaffolds led to more significant stimulation.
The magnetic scaffold can respond to the exterior magnetic field and engender some synergistic effect to intensify the stimulating effect of a magnetic field to the proliferation and differentiation of cells.
近年来，人们对用于组织工程的磁性仿生支架的兴趣大大增加。本研究组开发了一种由磁性纳米颗粒（MNPs）和羟基磷灰石（HA）组成的用于骨修复的磁性支架。
在这项研究中，为了研究 MNP 含量（支架中的）对细胞行为的影响以及磁性支架与外部磁场的相互作用，通过将 HA 支架浸入 MNP 胶体中，制备了一系列具有不同 MNP 含量（0.2%至 2%）的 MNP-HA 磁性支架。ROS 17/2.8 和 MC3T3-E1 细胞分别在有和没有外部磁场的情况下在支架上进行体外培养。通过扫描电子显微镜、共聚焦激光扫描显微镜以及 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）、碱性磷酸酶和骨钙蛋白活性试验评估细胞黏附、增殖和分化。
结果表明磁性支架对细胞黏附、增殖和分化有积极影响。此外，磁性支架上更多的 MNPs 导致更显著的刺激。
磁性支架可以对外界磁场做出响应，并产生一些协同效应，从而增强磁场对细胞增殖和分化的刺激作用。
