National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, People's Republic of China.
Biomed Mater. 2010 Feb;5(1):15001. doi: 10.1088/1748-6041/5/1/015001. Epub 2010 Jan 7.
A magnetic field has been applied to accelerate bone healing for a long time. In this study, in order to combine the bone repair capability of calcium phosphate (CaP) ceramics with the magnetic field, a novel CaP ceramic-magnetic nanoparticle (CaP-MNP) composite was fabricated through integrating the superparamagnetic nanoparticles into the CaP ceramics. Two kinds of CaP ceramics were chosen: hydroxyapatite (HA) and HA/tricalcium phosphate (65/35, HT). The samples were cultured with Ros17/2.8 and MG63 cells respectively in vitro to evaluate the cell proliferation and differentiation via MTT and alkaline phosphatase activity tests. In order to find the influence of the magnetic materials on the expression of the bone morphological protein (BMP), the samples composited with BMP-2 were implanted subcutaneously in the fasciae of rat back muscles for 30 days. Compared with ordinary CaP ceramics, the results indicated that the CaP-MNP composite had good biocompatibility and was able to promote cell proliferation and differentiation significantly. The in vivo test showed that the expression of BMP-2 would be accelerated by HT composited with MNPs, and new bone-like tissue formation could be observed. Accordingly, it might be expected that this CaP-MNP composite could become a potential bone substitute or bone tissue engineering scaffold.
磁场在促进骨愈合方面已经得到了长期的应用。在这项研究中，为了将磷酸钙（CaP）陶瓷的骨修复能力与磁场相结合，通过将超顺磁纳米粒子整合到 CaP 陶瓷中，制备了一种新型的 CaP 陶瓷-磁性纳米粒子（CaP-MNP）复合材料。选择了两种 CaP 陶瓷：羟基磷灰石（HA）和 HA/磷酸三钙（65/35，HT）。将样品分别与 Ros17/2.8 和 MG63 细胞在体外进行培养，通过 MTT 和碱性磷酸酶活性试验评估细胞增殖和分化。为了研究磁性材料对骨形态发生蛋白（BMP）表达的影响，将复合有 BMP-2 的样品植入大鼠背部肌肉筋膜下 30 天。与普通 CaP 陶瓷相比，结果表明 CaP-MNP 复合材料具有良好的生物相容性，并能显著促进细胞增殖和分化。体内试验表明，HT 与 MNPs 复合可加速 BMP-2 的表达，并可观察到新的类骨组织形成。因此，可以预期这种 CaP-MNP 复合材料可能成为一种有潜力的骨替代物或骨组织工程支架。
