Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden.
PLoS One. 2012;7(7):e41652. doi: 10.1371/journal.pone.0041652. Epub 2012 Jul 27.
Experimental and natural human adenovirus-36 (Adv36) infection of multiple animal species results in obesity through increasing adipogenesis and lipid accumulation in adipocytes. Presence of Adv36 antibodies detected by serum neutralization assay has previously been associated with obesity in children and adults living in the USA, South Korea and Italy, whereas no association with adult obesity was detected in Belgium/The Netherlands nor among USA military personnel. Adv36 infection has also been shown to reduce blood lipid levels, increase glucose uptake by adipose tissue and skeletal muscle biopsies, and to associate with improved glycemic control in non-diabetic individuals.
Using a novel ELISA, 1946 clinically well-characterized individuals including 424 children and 1522 non-diabetic adults, and 89 anonymous blood donors, residing in central Sweden representing the population in Stockholm area, were studied for the presence of antibodies against Adv36 in serum. The prevalence of Adv36 positivity in lean individuals increased from ∼7% in 1992-1998 to 15-20% in 2002-2009, which paralleled the increase in obesity prevalence. We found that Adv36-positive serology was associated with pediatric obesity and with severe obesity in females compared to lean and overweight/mildly obese individuals, with a 1.5 to 2-fold Adv36 positivity increase in cases. Moreover, Adv36 positivity was less common among females and males on antilipid pharmacological treatment or with high blood triglyceride level. Insulin sensitivity, measured as lower HOMA-IR, showed a higher point estimate in Adv36-positive obese females and males, although it was not statistically significant (p = 0.08).
Using a novel ELISA we show that Adv36 infection is associated with pediatric obesity, severe obesity in adult females and lower risk of high blood lipid levels in non-diabetic Swedish individuals.
实验和自然感染多种动物物种的人腺病毒-36（Adv36）会通过增加脂肪细胞中的脂肪生成和脂质积累导致肥胖。通过血清中和测定法检测到的 Adv36 抗体的存在先前与生活在美国、韩国和意大利的儿童和成年人肥胖有关，而在比利时/荷兰和美国军人中未检测到与成人肥胖有关。Adv36 感染还显示可降低血脂水平，增加脂肪组织和骨骼肌活检中的葡萄糖摄取，并与非糖尿病个体的血糖控制改善相关。
使用新型 ELISA，对包括 424 名儿童和 1522 名非糖尿病成年人在内的 1946 名临床特征良好的个体以及 89 名匿名献血者进行了研究，这些个体居住在瑞典中部，代表斯德哥尔摩地区的人群，以检测血清中针对 Adv36 的抗体。瘦个体中 Adv36 阳性的患病率从 1992-1998 年的约 7％增加到 2002-2009 年的 15-20％，这与肥胖症患病率的增加相吻合。我们发现 Adv36 阳性血清学与儿科肥胖以及女性严重肥胖有关，与瘦和超重/轻度肥胖个体相比，病例中 Adv36 阳性增加了 1.5 至 2 倍。此外，在接受抗脂类药物治疗或高甘油三酯水平的女性和男性中，Adv36 阳性的情况较少。Adv36 阳性肥胖的女性和男性的胰岛素敏感性（以较低的 HOMA-IR 表示）的点估计值更高，尽管没有统计学意义（p=0.08）。
使用新型 ELISA，我们表明 Adv36 感染与儿科肥胖，成年女性严重肥胖以及瑞典非糖尿病个体高血脂水平的风险降低有关。
