人类脂肪组织中的腺病毒36 DNA
Adenovirus 36 DNA in human adipose tissue.
作者信息
Ponterio E, Cangemi R, Mariani S, Casella G, De Cesare A, Trovato F M, Garozzo A, Gnessi L
机构信息
Department of Experimental Medicine, Section of Medical Pathophysiology Food Science and Endocrinology, Sapienza University of Rome, Rome, Italy.
Department of Internal Medicine and Medical Specialties, Sapienza University of Rome, Rome, Italy.
出版信息
Int J Obes (Lond). 2015 Dec;39(12):1761-4. doi: 10.1038/ijo.2015.163. Epub 2015 Aug 21.
Recent studies have suggested a possible correlation between obesity and adenovirus 36 (Adv36) infection in humans. As information on adenoviral DNA presence in human adipose tissue are limited, we evaluated the presence of Adv36 DNA in adipose tissue of 21 adult overweight or obese patients. Total DNA was extracted from adipose tissue biopsies. Virus detection was performed using PCR protocols with primers against specific Adv36 fiber protein and the viral oncogenic E4orf1 protein nucleotide sequences. Sequences were aligned with the NCBI database and phylogenetic analyses were carried out with MEGA6 software. Adv36 DNA was found in four samples (19%). This study indicates that some individuals carry Adv36 in the visceral adipose tissue. Further studies are needed to determine the specific effect of Adv36 infection on adipocytes, the prevalence of Adv36 infection and its relationship with obesity in the perspective of developing a vaccine that could potentially prevent or mitigate infection.
近期研究表明，人类肥胖与腺病毒36型（Adv36）感染之间可能存在关联。由于关于人类脂肪组织中腺病毒DNA存在情况的信息有限，我们评估了21名成年超重或肥胖患者脂肪组织中Adv36 DNA的存在情况。从脂肪组织活检样本中提取总DNA。使用针对特定Adv36纤维蛋白和病毒致癌E4orf1蛋白核苷酸序列的引物，通过PCR方案进行病毒检测。将序列与NCBI数据库进行比对，并使用MEGA6软件进行系统发育分析。在四个样本（19%）中发现了Adv36 DNA。这项研究表明，一些个体在内脏脂肪组织中携带Adv36。需要进一步研究以确定Adv36感染对脂肪细胞的具体影响、Adv36感染的患病率及其与肥胖的关系，从而开发出可能预防或减轻感染的疫苗。
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