Zhejiang Key Laboratory for Diagnosis and Therapy of neonatal Diseases, Key Laboratory of Reproductive Genetics (Zhejiang University) Ministry of Education, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
Arch Virol. 2013 Jan;158(1):173-7. doi: 10.1007/s00705-012-1378-8. Epub 2012 Aug 1.
Human cytomegalovirus (HCMV) has evolved several immune evasion strategies. One strategy is controlling the movement of peripheral blood mononuclear cells (PBMCs) by encoding homologues of chemokines. Our aim was to determine whether HCMV open reading frame (ORF) UL128 could encode a protein that attracts PBMCs like a β-chemokine. The recombinant UL128 protein was synthesized by construction of a stably transfected CHO-UL128 cell line, and a chemotaxis assay showed that UL128 was able to attract PBMCs with a potency equal to that of MIP-1α in vitro. We hypothesize that UL128 protein may act as a β-chemokine homologue in viral pathogenesis.
人类巨细胞病毒（HCMV）已经进化出几种免疫逃逸策略。一种策略是通过编码趋化因子的同源物来控制外周血单核细胞（PBMC）的运动。我们的目的是确定 HCMV 开放阅读框（ORF）UL128 是否可以编码一种像β-趋化因子一样吸引 PBMC 的蛋白。通过构建稳定转染的 CHO-UL128 细胞系合成重组 UL128 蛋白，趋化性测定表明 UL128 能够在体外以与 MIP-1α 相当的效力吸引 PBMC。我们假设 UL128 蛋白在病毒发病机制中可能作为β-趋化因子同源物发挥作用。
