Hirsch P, Rades-Rohkohl E
Institut für Allgemeine Mikrobiologie, Universität Kiel, Federal Republic of Germany.
Appl Environ Microbiol. 1990 Oct;56(10):2963-6. doi: 10.1128/aem.56.10.2963-2966.1990.
Microbial growth within the water-saturated subsurface environment was investigated by exposing sandy sediments to groundwater for 12 weeks at a depth of 10 or 20 m in a stainless-steel groundwater well. Washing and heating the sediment to 600 degrees C (removal of organic carbon) prior to the exposure did not prevent the natural microbial community from colonizing the sterilized sediment samples. Total cell counts of more than 10(7) or 10(8) per g of dried sediment were obtained. Viable cell counts of 10(5) cells per g on oligotrophic media indicated the presence, within the exposed sediment, of a highly active and multiplying biota. Microscopic analysis of enrichments inoculated with exposed sediment samples revealed a total of 45 different morphotypes, approximately 42% of the microbial community observed in previous studies of this site. The interstitial water running off of the retrieved sediment contained only 17 morphotypes and had up to 6 x 10(5) viable cells per ml.
通过将沙质沉积物置于不锈钢地下水井中10米或20米深处的地下水中12周，研究了水饱和地下环境中的微生物生长情况。在暴露之前，将沉积物洗涤并加热至600摄氏度（去除有机碳），这并没有阻止天然微生物群落定殖于经过灭菌的沉积物样品。每克干燥沉积物的总细胞数超过10⁷或10⁸。在贫营养培养基上每克有10⁵个活细胞的计数表明，在暴露的沉积物中存在高度活跃且正在繁殖的生物群。对接种了暴露沉积物样品的富集培养物进行显微镜分析，共发现了45种不同的形态类型，约占该地点先前研究中观察到的微生物群落的42%。从取回的沉积物中流出的间隙水仅含有17种形态类型，每毫升最多有6×10⁵个活细胞。
