Cornax R, Moriñigo M A, Paez I G, Muñoz M A, Borrego J J
Department of Microbiology, Faculty of Sciences, University of Malaga, Spain.
Appl Environ Microbiol. 1990 Oct;56(10):3170-3. doi: 10.1128/aem.56.10.3170-3173.1990.
The direct double-agar-layer plaque assay for the detection and enumeration of specific bacteriophages of Bacteroides fragilis from contaminated-water samples was performed. Several factors that affect the methods, such as conditions of the bacterial culture, composition of the assay medium, addition of divalent cations, and decontamination techniques applied to the sample, were evaluated. The results obtained show that the direct assay technique proved to be more efficient than the most-probable-number technique. A higher recovery of bacteriophages was obtained from 17 of 24 samples with the direct assay. The two methods only showed similar results from samples with a low degree of pollution.
采用直接双琼脂层噬菌斑试验对受污染水样中脆弱拟杆菌的特定噬菌体进行检测和计数。评估了几个影响该方法的因素，如细菌培养条件、试验培养基的组成、二价阳离子的添加以及应用于样品的去污技术。所得结果表明，直接试验技术比最大可能数技术更有效。通过直接试验，在24个样品中的17个样品中获得了更高的噬菌体回收率。这两种方法仅在污染程度较低的样品中显示出相似的结果。
