Armon R, Kott Y
Environmental and Water Resources Engineering, Technion, Haifa, Israel.
J Appl Bacteriol. 1993 Apr;74(4):490-6. doi: 10.1111/j.1365-2672.1993.tb05159.x.
A rapid, simple and sensitive direct bacteriophage presence detection method for 500 ml drinking water samples has been developed. The method includes a glass device consisting of a jar containing the water sample and an immersible probe filled with solidified soft agar containing bacterial host cells. Host bacteria in logarithmic phase were added to the experimental volume and the probe was submerged. The entire device was incubated in a water bath at 36 degrees C. Plaques of somatic bacteriophage infecting Escherichia coli strain CN13, could be detected within 3 h. Male-specific bacteriophages infecting E. coli F+ amp were detected within 6 h. Bacteriophage infecting the anaerobe Bacteroides fragilis subsp. fragilis HSP40 were detected after 8 h. Application of this device and the associated technique, enabled a one-step detection of 1 pfu of E. coli or Bact. fragilis specific bacteriophage in 500 ml drinking water samples.
已开发出一种用于检测500毫升饮用水样本中噬菌体存在的快速、简单且灵敏的直接检测方法。该方法包括一个玻璃装置，该装置由一个装有水样的广口瓶和一个装有含有细菌宿主细胞的凝固软琼脂的可浸入式探针组成。将对数期的宿主细菌添加到实验体积中，并将探针浸没。整个装置在36℃的水浴中孵育。感染大肠杆菌CN13菌株的体细胞噬菌体噬菌斑可在3小时内检测到。感染大肠杆菌F+ amp的雄性特异性噬菌体在6小时内被检测到。感染厌氧菌脆弱拟杆菌脆弱亚种HSP40的噬菌体在8小时后被检测到。应用该装置及相关技术，能够一步检测500毫升饮用水样本中1个噬菌斑形成单位的大肠杆菌或脆弱拟杆菌特异性噬菌体。
