Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
Biomacromolecules. 2012 Sep 10;13(9):2609-15. doi: 10.1021/bm3009445. Epub 2012 Aug 14.
Water-soluble poly(L-serine)s bearing long side-chain with terminal charge groups were synthesized via ring-opening polymerization of O-pentenyl-L-serine N-carboxyanhydride followed by thiol-ene reactions. These side-chain modified poly(L-serine)s adopt β-sheet conformation in aqueous solution with excellent stability against changes in pH and temperature. These water-soluble poly(L-serine) derivatives with charged side-chain functional groups and stable β-sheet conformations showed membrane-penetrating capabilities in different cell lines with low cytotoxicity.
通过 O-戊烯基-L-丝氨酸 N-羧酸酐的开环聚合，然后进行硫醇-烯反应，合成了带有末端电荷基团的长侧链的水溶性聚（L-丝氨酸）。这些侧链修饰的聚（L-丝氨酸）在水溶液中采用β-折叠构象，对 pH 和温度的变化具有优异的稳定性。这些带有电荷侧链官能团和稳定β-折叠构象的水溶性聚（L-丝氨酸）衍生物在具有低细胞毒性的不同细胞系中表现出膜穿透能力。
