翻译控制肿瘤蛋白（TCTP）存在于人类角膜中，并在疱疹性角膜炎中增加。
Translationally controlled tumour protein (TCTP) is present in human cornea and increases in herpetic keratitis.
机构信息
Department of Ophthalmology, University of Siena, Siena, Italy.
出版信息
Diagn Pathol. 2012 Aug 1;7:90. doi: 10.1186/1746-1596-7-90.
BACKGROUND
Translationally controlled tumour protein is a multifunctional calcium binding protein which has an important role in apoptosis, calcium levels balance and immunological response. The aim of this study was to evaluated the presence and distribution of TCTP in healthy human corneas and to identify and characterize the presence and distribution of this protein in human normal cornea. Since recent studies suggest that apoptosis, calcium levels and immunological mechanisms play a role in the pathogenesis of herpetic stromal keratitis, we studied TCTP expression in this disease.
METHODS
We evaluated the expression of TCTP at both RNA messanger and protein level by using reverse transcriptase analysis, immunoblotting and immunohistochemistry in 10 healthy samples cornea: four obtained after penetrating keratoplasty and six from eyes enucleated for other pathologies. Finally, we analysed by immunohistochemistry ten paraffin-embedded samples of Herpes simplex virus keratitis collected at Siena Department of Human Pathology and Oncology: 5 had clinically quiescent disease and 5 had active corneal inflammation.
RESULTS
Reverse transcriptase and immunoblotting demonstrated TCTP expression in cornea as a 22,000 Da molecular weight band corresponding to the molecular weight of this protein. Immunohistochemically, all the layers of normal corneal epithelium showed TCTP cytoplasmic expression. TCTP was, also, observed in keratocytes and in the endothelium. In Herpes simplex virus keratitis samples, strong expression of TCTP was evident in stromal cells, in the inflammatory infiltrate and in neo-vessels.
CONCLUSIONS
In this preliminary study we demonstrated, for the first time, the presence of TCTP in human cornea, suggesting a potential role in the pathogenesis of herpes virus keratitis.
VIRTUAL SLIDES
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/3306813447428149.
背景
翻译控制肿瘤蛋白是一种多功能钙结合蛋白，在细胞凋亡、钙平衡和免疫反应中具有重要作用。本研究旨在评估 TCTP 在健康人角膜中的存在和分布，并鉴定和描述这种蛋白在人正常角膜中的存在和分布。由于最近的研究表明，细胞凋亡、钙水平和免疫机制在单纯疱疹性基质性角膜炎的发病机制中起作用，我们研究了 TCTP 在这种疾病中的表达。
方法
我们使用逆转录分析、免疫印迹和免疫组织化学，在 10 例健康角膜样本（4 例取自穿透性角膜移植术，6 例取自因其他病理原因被摘除的眼球）中评估 TCTP 的 RNA 信使和蛋白质水平的表达。最后，我们通过免疫组织化学分析了来自锡耶纳人类病理学和肿瘤学系的 10 例单纯疱疹病毒角膜炎的石蜡包埋样本：5 例临床静止性疾病，5 例活动性角膜炎症。
结果
逆转录和免疫印迹显示 TCTP 在角膜中表达为 22000 Da 的分子量带，与该蛋白的分子量相对应。免疫组织化学显示，正常角膜上皮的所有层均显示 TCTP 细胞质表达。TCTP 也存在于角膜细胞和成纤维细胞中。在单纯疱疹病毒角膜炎样本中，基质细胞、炎症浸润和新生血管中 TCTP 的表达明显增强。
结论
在这项初步研究中，我们首次证明 TCTP 存在于人角膜中，提示其在单纯疱疹病毒角膜炎发病机制中具有潜在作用。
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