Research Center for Applied Sciences, Academia Sinica, 128 Sec. 2, Academia Rd. Nankang, Taipei, 11529, Taiwan.
Lab Chip. 2012 Oct 7;12(19):3552-65. doi: 10.1039/c2lc40509a.
Quantitative analysis of the output of processes and molecular interactions within a single cell is highly critical to the advancement of accurate disease screening and personalized medicine. Optical detection is one of the most broadly adapted measurement methods in biological and clinical assays and serves cellular phenotyping. Recently, microfluidics has obtained increasing attention due to several advantages, such as small sample and reagent volumes, very high throughput, and accurate flow control in the spatial and temporal domains. Optofluidics, which is the attempt to integrate optics with microfluidics, shows great promise to enable on-chip phenotypic measurements with high precision, sensitivity, specificity, and simplicity. This paper reviews the most recent developments of optofluidic technologies for cellular phenotyping optical detection.
对单个细胞内的过程和分子相互作用的输出进行定量分析，对于实现准确的疾病筛查和个性化医疗至关重要。光学检测是生物和临床分析中应用最广泛的测量方法之一，可用于细胞表型分析。最近，由于微流控技术具有样品和试剂用量小、高通量以及在时空域内实现精确流量控制等优点，引起了越来越多的关注。将光学与微流控相结合的光流控技术，有望实现高精度、高灵敏度、高特异性和简单易用的芯片上表型测量。本文综述了用于细胞表型光学检测的光流控技术的最新进展。
