Perrin P, Morgeaux S, Sureau P
Rabies Unit, Institut Pasteur, Paris, France.
Biologicals. 1990 Oct;18(4):321-30. doi: 10.1016/1045-1056(90)90037-z.
The replacement of the in vivo potency test (NIH test) for rabies vaccine evaluation by in vitro methods is at present discussed in many reports and also by WHO expert working groups. For this purpose, in vitro glycoprotein titration has been proposed. Among the different glycoprotein assays, we have studied two ELISA methods (immunocapture and direct plate coating with the antigen to be tested) using neutralizing mono- and polyclonal antibodies. In our view, the immunocapture method based on the use of a neutralizing monoclonal anti-glycoprotein antibody seems to be a convenient tool for the determination of the in vitro potency of rabies vaccine and of the products corresponding to the different steps of their production process.
目前，许多报告以及世界卫生组织专家工作组都在讨论用体外方法替代狂犬病疫苗评估的体内效力试验（NIH试验）。为此，有人提出了体外糖蛋白滴定法。在不同的糖蛋白检测方法中，我们研究了两种使用中和单克隆和多克隆抗体的ELISA方法（免疫捕获法和用待测抗原直接包被平板法）。我们认为，基于使用中和单克隆抗糖蛋白抗体的免疫捕获法似乎是测定狂犬病疫苗及其生产过程不同步骤相应产品体外效力的便捷工具。
